Yang Xin, Li Yunhui, Wang Yuxin, Wang Junwei, Lai Peng, Li Yuan, Song Junke, Qi Meng, Zhao Guanghui
College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China.
College of Animal Science, Tarim University, Alar 843300, China.
Animals (Basel). 2021 Oct 20;11(11):3016. doi: 10.3390/ani11113016.
sp. is an important zoonotic protist in humans and various animals with worldwide distribution. However, there have been no data on the occurrence of sp. in , an important economic animal in northwestern China. In the present study, a PCR-sequencing tool based on the SSU rRNA gene was applied to investigate the prevalence and genetic diversity of sp. in 638 faecal samples from in 21 sampling sites within three main breeding areas (Gansu, Inner Mongolia and Xinjiang) in northwestern China. The total prevalence of sp. was 21.8% (139/638) in , with the infection rates of 29.5% (18/61), 50.0% (14/28) and 19.5% (107/549) for animals aged <2 years, 2-6 years and >6 years, respectively. Significant differences in prevalence were detected among from three geographic areas (χ = 19.972, = 2, < 0.001) and all sampling sites (χ = 104.154, = 20, < 0.001). A total of 16 of 21 sampling sites were positive for sp., with the prevalence ranging from 7.7% to 70.6%. Sequence analysis of the SSU rRNA gene identified eight subtypes in in the present study, including seven animal adapted subtypes (ST10, ST14, ST21, ST24, ST25, ST26 and ST30) and one potentially novel subtype, with ST10 being the dominant one. To the best of our knowledge, this study provides the first insight for the occurrence and genetic make-up of sp. in and contributes to the understanding of the transmission of infection in in China.
[物种名称]是一种重要的人畜共患原生生物，在人类和各种动物中广泛分布于全球。然而，在中国西北部一种重要的经济动物[动物名称]中，尚无关于[物种名称]存在情况的数据。在本研究中，应用基于小亚基核糖体RNA（SSU rRNA）基因的PCR测序工具，对中国西北部三个主要养殖区（甘肃、内蒙古和新疆）21个采样点的638份[动物名称]粪便样本中[物种名称]的流行情况和遗传多样性进行了调查。[物种名称]在[动物名称]中的总流行率为21.8%（139/638），其中年龄<2岁、2 - 6岁和>6岁动物的感染率分别为29.5%（18/61）、50.0%（14/28）和19.5%（107/549）。在来自三个地理区域的[动物名称]中检测到流行率存在显著差异（χ² = 19.972，自由度 = 2，P < 0.001），在所有采样点中也存在显著差异（χ² = 104.154，自由度 = 20，P < 0.001）。21个采样点中有16个[物种名称]呈阳性，流行率范围为7.7%至70.6%。对SSU rRNA基因的序列分析在本研究的[动物名称]中鉴定出8个亚型，包括7个适应动物的亚型（ST10、ST14、ST21、ST24、ST25、ST26和ST30）和1个潜在的新亚型，其中ST10为优势亚型。据我们所知，本研究首次揭示了[物种名称]在[动物名称]中的发生情况和遗传组成，有助于了解中国[动物名称]中[物种名称]感染的传播情况。
