Krasztel Magdalena Maria, Czopowicz Michał, Szaluś-Jordanow Olga, Moroz Agata, Mickiewicz Marcin, Kaba Jarosław
Division of Veterinary Epidemiology and Economics, Institute of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159c, 02-776 Warsaw, Poland.
Department of Small Animal Diseases with Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159c, 02-776 Warsaw, Poland.
Animals (Basel). 2021 Nov 6;11(11):3172. doi: 10.3390/ani11113172.
The colorimetric catalytic assay based on the use of 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6'-methylresorufin) (DGGR) ester as a substrate for pancreatic lipase activity is commonly used for the diagnosis of pancreatitis in dogs and cats. Even though the assay has generally been shown to yield consistent results with feline pancreatic lipase immunoreactivity (fPLI) assay, the agreement may vary between assays of different manufacturers. In this study, the chance-corrected agreement between a DGGR-lipase assay offered by one of the biggest providers of diagnostic solutions in Poland and fPLI assay was investigated. The study was carried out on 50 cats in which DGGR-lipase activity and fPLI were tested in the same blood sample. The chance-corrected agreement was determined using Gwet's AC coefficient separately for the fPLI assay's cut-off values of >3.5 μg/L and >5.3 μg/L. The DGGR-lipase activity significantly positively correlated with fPLI (R = 0.665; CI 95%: 0.451, 0.807, < 0.001). The chance-corrected agreement between the fPLI assay and DGGR-lipase assay differed considerably depending on the cut-off values of the DGGR-lipase assay. When the cut-off value reported in the literature (>26 U/L) was used, it was poor to fair. It was moderate at the cut-off value recommended by the laboratory (>45 U/L), and good at the cut-off value recommended by the assay's manufacturer (>60 U/L). The highest agreement was obtained between the fPLI assay at the cut-off value of 3.5 μg/L and the DGGR-lipase assay at the cut-off value of 55 U/L (AC = 0.725; CI 95%: 0.537, 0.914) and between the fPLI assay at the cut-off value of 5.3 μg/L and the DGGR-lipase assay at the cut-off value of 70 U/L (AC = 0.749; CI 95%: 0.577, 0.921). The study confirms that the chance-corrected agreement between the two assays is good. Prospective studies comparing both assays to a diagnostic gold standard are needed to determine which of them is more accurate.
基于使用1,2 - o - 二月桂酰 - rac - 甘油 - 3 - 戊二酸 - (6'-甲基试卤灵)(DGGR)酯作为胰腺脂肪酶活性底物的比色催化测定法常用于犬猫胰腺炎的诊断。尽管该测定法通常已被证明与猫胰腺脂肪酶免疫反应性(fPLI)测定法产生一致的结果，但不同制造商的测定法之间的一致性可能会有所不同。在本研究中，调查了波兰最大的诊断解决方案提供商之一提供的DGGR - 脂肪酶测定法与fPLI测定法之间的机遇校正一致性。该研究对50只猫进行，在同一血液样本中检测DGGR - 脂肪酶活性和fPLI。分别针对fPLI测定法>3.5 μg/L和>5.3 μg/L的临界值，使用Gwet's AC系数确定机遇校正一致性。DGGR - 脂肪酶活性与fPLI显著正相关(R = 0.665；95%置信区间：0.451, 0.807，< 0.001)。fPLI测定法与DGGR - 脂肪酶测定法之间的机遇校正一致性根据DGGR - 脂肪酶测定法的临界值有很大差异。当使用文献报道的临界值(>26 U/L)时，一致性为差到一般。在实验室推荐的临界值(>45 U/L)时为中等，在测定法制造商推荐的临界值(>60 U/L)时为良好。在临界值为3.5 μg/L的fPLI测定法与临界值为55 U/L的DGGR - 脂肪酶测定法之间(AC = 0.725；95%置信区间：0.537, 0.914)以及在临界值为5.3 μg/L的fPLI测定法与临界值为70 U/L的DGGR - 脂肪酶测定法之间(AC = 0.749；95%置信区间：0.577, 0.921)获得了最高的一致性。该研究证实两种测定法之间的机遇校正一致性良好。需要进行前瞻性研究，将两种测定法与诊断金标准进行比较，以确定哪种测定法更准确。
