1,2-二-月桂酰-消旋甘油-3-戊二酸-(6'-甲基试卤灵)酯（DGGR）脂肪酶预测犬胰腺特异性脂肪酶（cPL）的准确性及两种检测方法对犬急性胰腺炎的诊断准确性

Accuracy of 1,2-o-Dilauryl-rac-glycero-3-glutaric Acid-(6'-methylresorufin) Ester (DGGR)-Lipase to Predict Canine Pancreas-Specific Lipase (cPL) and Diagnostic Accuracy of Both Tests for Canine Acute Pancreatitis.

作者信息

Wolfer Lina A, Howard Judith, Peters Laureen M

机构信息

Clinical Diagnostic Laboratory, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland.

Division of Small Animal Internal Medicine, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland.

出版信息

Vet Sci. 2022 Apr 8;9(4):177. doi: 10.3390/vetsci9040177.

Different lipase assays have variable reported diagnostic accuracies for acute pancreatitis (AP) in dogs. The aims of this retrospective study were to evaluate optimal cutoffs for 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6′-methylresorufin) ester (DGGR)-lipase to predict diagnostic cutoffs of canine pancreas-specific lipase (cPL; IDEXX). DGGR-lipase activity and cPL from the same blood draw in 301 dogs with a variety of diseases were compared using Spearman’s rank correlation, Cohen’s kappa agreement, and receiver operating characteristic (ROC) curves. Activity of DGGR-lipase (10−15,616 U/L) and cPL concentrations (8.1−≥2000 µg/L) were highly correlated (rs = 0.91). Areas under the ROC curves (AUCs) to predict cPL >200 and ≥400 µg/L with DGGR-lipase were 0.97 and 0.99, with optimal cutoffs of 143 U/L (sensitivity (Se) 91.7%; specificity (Sp) 95.3%) and 205 U/L (Se 97.5%; Sp 96.4%), and Cohen’s kappa agreements of 0.87 and 0.92, respectively. AUCs for a clinical diagnosis of AP, assigned to 87/301 dogs, with DGGR-lipase (0.75) and cPL (0.76) did not differ significantly (p = 0.48); optimal cutoffs were 161 U/L for DGGR (Se 67%; Sp 81%) and 235 µg/L for cPL (Se 68%; Sp 84%). To conclude, DGGR-lipase is a highly accurate predictor of cPL with a comparable performance when used to diagnose AP in dogs.

不同的脂肪酶检测方法对犬急性胰腺炎（AP）的诊断准确性报道各异。本回顾性研究的目的是评估1,2 - O - 二月桂酰 - rac - 甘油 - 3 - 戊二酸 -（6′ - 甲基试卤灵）酯（DGGR）-脂肪酶的最佳临界值，以预测犬胰腺特异性脂肪酶（cPL；IDEXX）的诊断临界值。使用Spearman秩相关、Cohen's kappa一致性分析以及受试者工作特征（ROC）曲线，对301只患有各种疾病的犬同一血样中的DGGR - 脂肪酶活性和cPL进行了比较。DGGR - 脂肪酶活性（10−15,616 U/L）与cPL浓度（8.1−≥2000 µg/L）高度相关（rs = 0.91）。用DGGR - 脂肪酶预测cPL >200和≥400 µg/L时，ROC曲线下面积（AUCs）分别为0.97和0.99，最佳临界值分别为143 U/L（灵敏度（Se）91.7%；特异性（Sp）95.3%）和205 U/L（Se 97.5%；Sp 96.4%），Cohen's kappa一致性分别为0.87和0.92。对于临床诊断为AP的87/301只犬，DGGR - 脂肪酶（0.75）和cPL（0.76）的AUCs无显著差异（p = 0.48）；DGGR的最佳临界值为161 U/L（Se 67%；Sp 81%），cPL为235 µg/L（Se 68%；Sp 84%）。总之，DGGR - 脂肪酶是cPL的高度准确预测指标，在用于诊断犬AP时具有可比的性能。