Ding Qiang, Jin Miaohan, Kalds Peter, Meng Chunhua, Wang Huili, Zhong Jifeng, Wang Xiaolong, Chen Yulin
Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China.
Animals (Basel). 2021 Nov 8;11(11):3190. doi: 10.3390/ani11113190.
Extracellular vesicles (EVs), which exist in the follicular fluid of ruminant ovaries, are considered as cargo carriers for the transfer of biomolecules to recipient cells. However, the functions and changes in EVs in antral follicles remain ambiguous. In the present study, we isolated and characterized EVs from goat follicular fluid by means of differential ultracentrifugation and Western blotting of marker proteins. Bioinformatics tools were used to detect miRNA expression levels in EVs. Different miRNA expression patterns of EVs exist in small to large follicles. Thirteen differentially expressed miRNAs (seven upregulated and six downregulated) were identified and used for analysis. A total of 1948 predicted target genes of 13 miRNAs were mapped to signaling pathways, and three significantly enriched pathways (FoxO, MAPK, and PI3K-AKT signaling pathways) were involved in follicular development, as revealed by KEGG enrichment analysis. Our findings suggest that EVs in follicular fluid play biofunctional roles during follicular development in goats.
存在于反刍动物卵巢卵泡液中的细胞外囊泡（EVs）被认为是生物分子向受体细胞转移的载体。然而，窦状卵泡中EVs的功能和变化仍不明确。在本研究中，我们通过差速超速离心和标记蛋白的蛋白质印迹法从山羊卵泡液中分离并鉴定了EVs。利用生物信息学工具检测EVs中的miRNA表达水平。小卵泡到大卵泡中存在不同的EVs miRNA表达模式。鉴定出13个差异表达的miRNA（7个上调和6个下调）并用于分析。KEGG富集分析显示，13个miRNA的1948个预测靶基因被映射到信号通路，其中三个显著富集的通路（FoxO、MAPK和PI3K-AKT信号通路）参与卵泡发育。我们的研究结果表明，卵泡液中的EVs在山羊卵泡发育过程中发挥着生物功能作用。
