Choi Hyejin, Mun Daye, Ryu Sangdon, Kwak Min-Jin, Kim Bum-Keun, Park Dong-Jun, Oh Sangnam, Kim Younghoon
Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Korea.
Korea Food Research Institute, Wanju 55365, Korea.
J Anim Sci Technol. 2023 May;65(3):652-663. doi: 10.5187/jast.2022.e124. Epub 2023 May 31.
The rumen fluids contain a wide range of bacteria, protozoa, fungi, and viruses. The various ruminal microorganisms in the rumen provide nutrients by fermenting the forage they eat. During metabolic processes, microorganisms present in the rumen release diverse vesicles during the fermentation process. Therefore, in this study, we confirmed the function of rumen extracellular vesicles (EVs) and their interaction with the host. We confirmed the structure of the rumen EVs by transmission electron microscope (TEM) and the size of the particles using nanoparticle tracking analysis (NTA). Rumen EVs range in size from 100 nm to 400 nm and are composed of microvesicles, microparticles, and ectosomes. Using the smart animal model, we verified the interaction between the host and rumen EVs. Exposure of to rumen EVs did not significantly enhance longevity, whereas exposure to the pathogenic bacteria O157:H7 and significantly increased lifespan. Furthermore, transcriptome analysis showed gene expression alterations in exposed to rumen EVs, with significant changes in the metabolic pathway, fatty acid degradation, and biosynthesis of cofactors. Our study describes the effect of rumen EV interactions with the host and provides novel insights for discovering biotherapeutic agents in the animal industry.
瘤胃液中含有多种细菌、原生动物、真菌和病毒。瘤胃中的各种瘤胃微生物通过发酵它们所吃的草料来提供营养。在代谢过程中，瘤胃中存在的微生物在发酵过程中释放出各种囊泡。因此，在本研究中，我们证实了瘤胃细胞外囊泡（EVs）的功能及其与宿主的相互作用。我们通过透射电子显微镜（TEM）确认了瘤胃EVs的结构，并使用纳米颗粒跟踪分析（NTA）确定了颗粒的大小。瘤胃EVs的大小范围为100纳米至400纳米，由微囊泡、微粒和外泌体组成。使用智能动物模型，我们验证了宿主与瘤胃EVs之间的相互作用。暴露于瘤胃EVs并未显著延长寿命，而暴露于致病性细菌O157:H7则显著延长了寿命。此外，转录组分析显示，暴露于瘤胃EVs的[具体对象]中基因表达发生了改变，代谢途径、脂肪酸降解和辅因子生物合成方面有显著变化。我们的研究描述了瘤胃EVs与宿主相互作用的影响，并为在动物产业中发现生物治疗剂提供了新的见解。
