Podhorec Peter, Knowles Jindřiška, Vysloužil Jakub, Boryshpolets Sergii, Kubová Kateřina, Rodina Marek, Kholodnyy Vitaliy, Sotnikov Anatolii, Gela David, Dzyuba Borys
South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic.
Department of Pharmaceutical Technology, Faculty of Pharmacy, Masaryk University, Palackeho trida 1946/1, 612 00 Brno, Czech Republic.
Animals (Basel). 2021 Nov 19;11(11):3305. doi: 10.3390/ani11113305.
Carp pituitary treatment versus poly (lactiac-co-glycolic acid) microparticles with slow release of Alarelin at 35 µg kg or 200 µg kg body weight to induce spermiation was compared in sterlet . All hormone treatments initially increased testosterone and 11-ketotestosterone, with a subsequent decline in testosterone but consistent high levels of 11-ketotestosterone at 48 and 72 h post-treatment. Spermiation did not differ between hormone-treated groups, and was not detected in controls receiving saline solution. Administration of the carp pituitary led to maximum sperm production 24 h post-treatment, followed by a decrease at 48 h post-treatment, with no sperm obtained at 72 h. The effect of Alarelin at 35 µg kg bw and carp pituitary did not differ at 24 and 48 h post-treatment, whereas 200 µg kg bw Alarelin was associated with significantly lower spermatozoon concentration 24 h post-treatment compared to carp pituitary, with no difference in milt volume. Higher relative sperm production was observed 48 h after injection of Alarelin at 200 µg kg bw compared to carp pituitary. Spermatozoon motility was significantly higher in fish receiving Alarelin at 35 µg kg bw than 200 µg kg bw. The treatment with optimal effect on inducing spermiation was poly (lactic-co-glycolic acid) microparticles with slow release of Alarelin at 35 µg kg bw.
在小体鲟中比较了鲤鱼垂体处理与聚（乳酸 - 乙醇酸）微粒以35μg/kg或200μg/kg体重缓慢释放阿拉瑞林诱导排精的效果。所有激素处理最初均使睾酮和11 - 酮睾酮增加，随后睾酮下降，但在处理后48小时和72小时11 - 酮睾酮水平持续较高。激素处理组之间的排精情况无差异，接受盐溶液的对照组未检测到排精。注射鲤鱼垂体后24小时精子产量最高，随后在处理后48小时下降，在72小时未获得精子。在处理后24小时和48小时，35μg/kg体重的阿拉瑞林与鲤鱼垂体的效果无差异，而与鲤鱼垂体相比，200μg/kg体重的阿拉瑞林在处理后24小时精子浓度显著较低，精液体积无差异。与鲤鱼垂体相比，注射200μg/kg体重阿拉瑞林后48小时观察到相对较高的精子产量。接受35μg/kg体重阿拉瑞林的鱼精子活力显著高于接受200μg/kg体重阿拉瑞林的鱼。对诱导排精效果最佳的处理是聚（乳酸 - 乙醇酸）微粒以35μg/kg体重缓慢释放阿拉瑞林。
