• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

巴西亚马逊五个不同地区田间分离株中富含半胱氨酸的保护性抗原（PvCyRPA）的遗传多样性。

Genetic Diversity of Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon.

机构信息

Laboratory of Immunoparasitology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro 21040-900, Brazil.

Laboratory of Basic and Applied Immunology, Arthur Sá Earp Neto University Center, Petrópolis 25680-120, Brazil.

出版信息

Genes (Basel). 2021 Oct 21;12(11):1657. doi: 10.3390/genes12111657.

DOI:10.3390/genes12111657
PMID:34828264
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8623135/
Abstract

The Cysteine-Rich Protective Antigen (PvCyRPA) has an important role in erythrocyte invasion and has been considered a target for malaria vaccine development. Nonetheless, its genetic diversity remains uncharted in Brazilian malaria-endemic areas. Therefore, we investigated the genetic polymorphism in 98 field isolates from the Brazilian Amazon and its impact on the antigenicity of predicted B-cell epitopes. Genetic diversity parameters, population genetic analysis, neutrality test and the median-joining network were analyzed, and the potential amino acid polymorphism participation in B-cell epitopes was investigated. One synonymous and 26 non-synonymous substitutions defined fifty haplotypes. The nucleotide diversity and Tajima's D values varied across the coding gene. The exon-1 sequence had greater diversity than those of exon-2. Concerning the prediction analysis, seven sequences were predicted as linear B cell epitopes, the majority contained in conformational epitopes. Moreover, important amino acid polymorphism was detected in regions predicted to contain residues participating in B-cell epitopes. Our data suggest that the gene presents a moderate polymorphism in the studied isolates and such polymorphisms alter amino acid sequences contained in potential B cell epitopes, an important observation considering the antigen potentiality as a vaccine candidate to cover distinct endemic areas worldwide.

摘要

富含半胱氨酸的保护性抗原（PvCyRPA）在红细胞入侵中具有重要作用，已被认为是疟疾疫苗开发的一个目标。尽管如此，其遗传多样性在巴西疟疾流行地区仍未被发现。因此，我们研究了来自巴西亚马逊地区的 98 个现场分离株中的遗传多态性及其对预测 B 细胞表位抗原性的影响。分析了遗传多样性参数、群体遗传分析、中性检验和中位数连接网络，并研究了潜在的氨基酸多态性参与 B 细胞表位的情况。一个同义突变和 26 个非同义突变定义了 50 个单倍型。编码基因的核苷酸多样性和 Tajima's D 值各不相同。外显子 1 的序列比外显子 2 的序列多样性更大。关于预测分析，有七个序列被预测为线性 B 细胞表位，其中大多数包含在构象表位中。此外，在预测含有参与 B 细胞表位的残基的区域中检测到重要的氨基酸多态性。我们的数据表明，在所研究的分离株中，该基因存在中度的多态性，这种多态性改变了潜在 B 细胞表位中包含的氨基酸序列，这一观察结果对于作为疫苗候选物的抗原潜力考虑到在全球不同的流行地区具有重要意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6ac/8623135/599d17c884d6/genes-12-01657-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6ac/8623135/7a3c9d595107/genes-12-01657-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6ac/8623135/44a200e9599c/genes-12-01657-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6ac/8623135/09e23d168ba0/genes-12-01657-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6ac/8623135/599d17c884d6/genes-12-01657-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6ac/8623135/7a3c9d595107/genes-12-01657-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6ac/8623135/44a200e9599c/genes-12-01657-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6ac/8623135/09e23d168ba0/genes-12-01657-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6ac/8623135/599d17c884d6/genes-12-01657-g004.jpg

相似文献

1
Genetic Diversity of Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon.巴西亚马逊五个不同地区田间分离株中富含半胱氨酸的保护性抗原（PvCyRPA）的遗传多样性。
Genes (Basel). 2021 Oct 21;12(11):1657. doi: 10.3390/genes12111657.
2
Cysteine-Rich Protective Antigen Polymorphism at Exon-1 Shows Recombination and Signatures of Balancing Selection.外显子1处富含半胱氨酸的保护性抗原多态性显示出重组和平衡选择的特征。
Genes (Basel). 2020 Dec 28;12(1):29. doi: 10.3390/genes12010029.
3
Limited genetic diversity in the global Plasmodium vivax Cell traversal protein of Ookinetes and Sporozoites (CelTOS) sequences; implications for PvCelTOS-based vaccine development.全球间日疟原虫动合子和子孢子细胞穿越蛋白（CelTOS）序列的遗传多样性有限；对基于间日疟原虫CelTOS的疫苗开发的影响。
Infect Genet Evol. 2017 Sep;53:239-247. doi: 10.1016/j.meegid.2017.06.005. Epub 2017 Jun 6.
4
Population genetic structure and polymorphism analysis of gene encoding apical membrane antigen-1 (AMA-1) of Iranian Plasmodium vivax wild isolates.伊朗间日疟原虫野毒株顶膜蛋白 1（AMA-1）基因的种群遗传结构和多态性分析。
Acta Trop. 2013 Jun;126(3):269-79. doi: 10.1016/j.actatropica.2013.02.017. Epub 2013 Mar 4.
5
Plasmodium vivax ookinete surface protein (Pvs25) is highly conserved among field isolates from five different regions of the Brazilian Amazon.恶性疟原虫动合子表面蛋白（Pvs25）在巴西亚马逊地区五个不同地区的野外分离株中高度保守。
Infect Genet Evol. 2019 Sep;73:287-294. doi: 10.1016/j.meegid.2019.05.003. Epub 2019 May 8.
6
Global Population Structure of the Genes Encoding the Malaria Vaccine Candidate, Plasmodium vivax Apical Membrane Antigen 1 (PvAMA1).编码疟疾候选疫苗抗原 1（PvAMA1）的基因的全球人群结构。
PLoS Negl Trop Dis. 2013 Oct 31;7(10):e2506. doi: 10.1371/journal.pntd.0002506. eCollection 2013.
7
Global genetic diversity of the Plasmodium vivax transmission-blocking vaccine candidate Pvs48/45.间日疟原虫传播阻断疫苗候选物Pvs48/45的全球遗传多样性
Malar J. 2016 Apr 12;15:202. doi: 10.1186/s12936-016-1263-0.
8
Population genetics and natural selection in the gene encoding the Duffy binding protein II in Iranian Plasmodium vivax wild isolates.伊朗间日疟原虫野生分离株中编码达菲结合蛋白II的基因的群体遗传学与自然选择
Infect Genet Evol. 2014 Jan;21:424-35. doi: 10.1016/j.meegid.2013.12.012. Epub 2013 Dec 30.
9
Diversity pattern of Duffy binding protein sequence among Duffy-negatives and Duffy-positives in Sudan.在苏丹的 Duffy 阴性和 Duffy 阳性个体中，Duffy 结合蛋白序列的多样性模式。
Malar J. 2018 Aug 17;17(1):297. doi: 10.1186/s12936-018-2425-z.
10
Evaluation of the genetic diversity of domain II of Plasmodium vivax Apical Membrane Antigen 1 (PvAMA-1) and the ensuing strain-specific immune responses in patients from Sri Lanka.评估来自斯里兰卡患者的恶性疟原虫顶膜蛋白 1（PvAMA-1）结构域 II 的遗传多样性以及由此产生的针对特定株系的免疫应答。
Vaccine. 2011 Oct 6;29(43):7491-504. doi: 10.1016/j.vaccine.2011.07.029. Epub 2011 Jul 23.

引用本文的文献

1
Characterization of T and B cell epitopes in PvCyRPA by studying the naturally acquired immune response in Brazilian Amazon communities.通过研究巴西亚马逊社区中自然获得的免疫反应来描绘 PvCyRPA 中的 T 细胞和 B 细胞表位。
Sci Rep. 2024 Nov 9;14(1):27343. doi: 10.1038/s41598-024-72671-x.
2
Immunogenicity of PvCyRPA, PvCelTOS and Pvs25 chimeric recombinant protein of in murine model.在小鼠模型中对 PvCyRPA、PvCelTOS 和 Pvs25 嵌合重组蛋白的免疫原性研究。
Front Immunol. 2024 Jun 19;15:1392043. doi: 10.3389/fimmu.2024.1392043. eCollection 2024.
3
Construction, Expression, and Evaluation of the Naturally Acquired Humoral Immune Response against RMC-1, a Multistage Chimeric Protein.

本文引用的文献

1
Using Plasmodium knowlesi as a model for screening Plasmodium vivax blood-stage malaria vaccine targets reveals new candidates.利用疟原虫 knowlesi 作为筛选间日疟原虫血期疟疾疫苗靶点的模型揭示了新的候选者。
PLoS Pathog. 2021 Jul 1;17(7):e1008864. doi: 10.1371/journal.ppat.1008864. eCollection 2021 Jul.
2
Cysteine-Rich Protective Antigen Polymorphism at Exon-1 Shows Recombination and Signatures of Balancing Selection.外显子1处富含半胱氨酸的保护性抗原多态性显示出重组和平衡选择的特征。
Genes (Basel). 2020 Dec 28;12(1):29. doi: 10.3390/genes12010029.
3
Population genomics reveals the expansion of highly inbred Plasmodium vivax lineages in the main malaria hotspot of Brazil.
针对 RMC-1（一种多阶段嵌合蛋白）的天然获得性体液免疫反应的构建、表达和评估。
Int J Mol Sci. 2023 Jul 18;24(14):11571. doi: 10.3390/ijms241411571.
人群基因组学揭示了高度近亲繁殖的间日疟原虫系在巴西主要疟疾热点地区的扩张。
PLoS Negl Trop Dis. 2020 Oct 28;14(10):e0008808. doi: 10.1371/journal.pntd.0008808. eCollection 2020 Oct.
4
Human Plasmodium vivax diversity, population structure and evolutionary origin.人类间日疟原虫的多样性、种群结构和进化起源。
PLoS Negl Trop Dis. 2020 Mar 9;14(3):e0008072. doi: 10.1371/journal.pntd.0008072. eCollection 2020 Mar.
5
Vaccination with virosomally formulated recombinant CyRPA elicits protective antibodies against parasites in preclinical in vitro and in vivo models.在临床前体外和体内模型中，用病毒体配方的重组CyRPA进行疫苗接种可引发针对寄生虫的保护性抗体。
NPJ Vaccines. 2020 Jan 31;5:9. doi: 10.1038/s41541-020-0158-9. eCollection 2020.
6
Spatial spread of malaria and economic frontier expansion in the Brazilian Amazon.巴西亚马逊地区疟疾的空间传播与经济前沿的扩张。
PLoS One. 2019 Jun 18;14(6):e0217615. doi: 10.1371/journal.pone.0217615. eCollection 2019.
7
Divergent roles for the RH5 complex components, CyRPA and RIPR in human-infective malaria parasites.RH5 复合物成分 CyRPA 和 RIPR 在人类感染性疟原虫中的不同作用。
PLoS Pathog. 2019 Jun 11;15(6):e1007809. doi: 10.1371/journal.ppat.1007809. eCollection 2019 Jun.
8
Plasmodium vivax ookinete surface protein (Pvs25) is highly conserved among field isolates from five different regions of the Brazilian Amazon.恶性疟原虫动合子表面蛋白（Pvs25）在巴西亚马逊地区五个不同地区的野外分离株中高度保守。
Infect Genet Evol. 2019 Sep;73:287-294. doi: 10.1016/j.meegid.2019.05.003. Epub 2019 May 8.
9
Plasmodium genomics: an approach for learning about and ending human malaria.疟原虫基因组学：一种了解并终结人类疟疾的方法。
Parasitol Res. 2019 Jan;118(1):1-27. doi: 10.1007/s00436-018-6127-9. Epub 2018 Nov 6.
10
Molecular and cellular interactions defining the tropism of Plasmodium vivax for reticulocytes.定义间日疟原虫对网织红细胞嗜性的分子和细胞相互作用。
Curr Opin Microbiol. 2018 Dec;46:109-115. doi: 10.1016/j.mib.2018.10.002. Epub 2018 Oct 23.