Xiang Wei, Xia Zhining, Xu Li
School of Pharmaceutical Sciences, Chongqing University, Chongqing 401331, China.
State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing 400715, China.
Foods. 2021 Nov 2;10(11):2659. doi: 10.3390/foods10112659.
As a by-product in the sericulture industry, mulberry branches are not currently utilized effectively. Jialing 20 is an artificial triploids mulberry that widely cultivated in southwest China. In this study, the chemical composition of the Jialing 20 mulberry branch extract (MBE) was first analyzed by UPLC-MS/MS, and 42 components, including alkaloids, flavonoids, and coumarins, were obtained. Then, the antioxidant activities, hypoglycemic effect, Alzheimer's disease inhibition, and cardiovascular protection of MBE were also evaluated in vitro. The IC values for the scavenging DPPH and ABTS radicals were, respectively, 31.23 ± 0.57 μg/mL and 8.88 ± 0.36 μg/mL (IC values of positive Vc were, respectively, 4.41 ± 0.19 μg/mL and 8.79 ± 0.41 μg/mL). The IC value for inhibiting α-glucosidase was 1.90 ± 0.05 μg/mL (IC value of positive acarbose was 0.03 μg/mL). The IC values for inhibiting acetylcholinesterase and butyrylcholinesterase were, respectively, 179.47 ± 0.38 μg/mL and 101.82 ± 3.37 μg/mL (IC values of positive berberine were, respectively, 1.27 ± 0.03 μg/mL and 57.41 ± 0.21 μg/mL). MBE (10 μg/mL and 40 μg/mL) significantly increased the survival rate of oxidized low-density lipoprotein- (ox-LDL) induced human umbilical vein endothelial cells (HUVECs) and significantly decreased the intracellular reactive oxygen species. These results suggest that the extracts of Jialing 20 mulberry branches could be used as a functional food additive.
作为蚕桑产业的副产品，桑树枝目前未得到有效利用。嘉陵20是一种在中国西南地区广泛种植的人工三倍体桑树。本研究首先通过超高效液相色谱-串联质谱法（UPLC-MS/MS）分析了嘉陵20桑树枝提取物（MBE）的化学成分，共获得42种成分，包括生物碱、黄酮类化合物和香豆素类。然后，还在体外评估了MBE的抗氧化活性、降血糖作用、对阿尔茨海默病的抑制作用以及心血管保护作用。清除DPPH和ABTS自由基的IC值分别为31.23±0.57μg/mL和8.88±0.36μg/mL（阳性对照维生素C的IC值分别为4.41±0.19μg/mL和8.79±0.41μg/mL）。抑制α-葡萄糖苷酶的IC值为1.90±0.05μg/mL（阳性对照阿卡波糖的IC值为0.03μg/mL）。抑制乙酰胆碱酯酶和丁酰胆碱酯酶的IC值分别为179.47±0.38μg/mL和101.82±3.37μg/mL（阳性对照黄连素的IC值分别为1.27±0.03μg/mL和57.41±0.21μg/mL）。MBE（10μg/mL和40μg/mL）显著提高了氧化型低密度脂蛋白（ox-LDL）诱导的人脐静脉内皮细胞（HUVECs）的存活率，并显著降低了细胞内活性氧水平。这些结果表明，嘉陵20桑树枝提取物可作为功能性食品添加剂。
