沙枣精油的化学成分、体外抗氧化、乙酰胆碱酯酶抑制和抗糖尿病活性研究。

Chemical composition, in vitro antioxidant, anticholinesterase, and antidiabetic potential of essential oil of Elaeagnus umbellata Thunb.

机构信息

Department of Biochemistry, University of Malakand, Chakdara Dir (L), Khyber Pakhtunkhwa, Pakistan.

Department of Electrical Engineering, Sarhad University of Science and Information Technology, Peshawar, Pakistan.

出版信息

BMC Complement Med Ther. 2021 Feb 22;21(1):73. doi: 10.1186/s12906-021-03228-y.

BACKGROUND

Elaeagnus umbellata Thunb. (autumn olive) is a high valued medicinal plant. It belongs to Elaeagnaceae family and is widely distributed in Himalayan regions of Pakistan. In the present study essential oil were extracted from the fruit of this plant and their antioxidant, anticholinesterase and antidiabetic potentials were also evaluated.

METHODS

Essential oils were extracted from the fruit of E. umbellata using hydro-distillation method and were characterized by GC-MS. The extracted oil were tested for its antioxidant, anticholinesterase, and antidiabetic potentials using standard protocols.

RESULTS

About 68 compounds were identified by GC-MS. The extracted oil exhibited a fairly high free radical scavenging activities against DPPH and ABTS radicals with IC values of 70 and 105 μg/mL respectively (for ascorbic acid, used as standard, the IC values were 32 and 29 μg/mL, respectively against the mentioned radicals). The essential oil also exhibited anticholinesterase activities with IC values of 48 and 90 μg/mL respectively against AChE and BChE (for galantamine used as standard, the IC values were 25 and 30 μg/mL respectively). The essential oil also exhibited antidiabetic potential with IC values of 120 and 110 μg/mL respectively against α-glucosidase and α-amylase (IC values for standard acarbose = 28 and 30 μg/mL respectively).

CONCLUSION

Essential oil extracted from the fruits of E. umbellata exhibited reasonable antioxidant, anticholinesterase, and antidiabetic potentials that could be used as alternative medicine in treating diabetes and neurodegenerative disorders. However, further studies are needed to isolate responsible compounds and evaluate the observed potential in animal models.

背景

翅果油树（Autumn Olive）是一种具有高价值的药用植物。它属于胡颓子科，广泛分布于巴基斯坦喜马拉雅地区。本研究从该植物的果实中提取了精油，并评估了其抗氧化、抗胆碱酯酶和抗糖尿病潜力。

方法

采用水蒸馏法从翅果油树的果实中提取精油，并通过 GC-MS 进行特征分析。使用标准方案测试提取油的抗氧化、抗胆碱酯酶和抗糖尿病潜力。

结果

通过 GC-MS 鉴定出约 68 种化合物。该提取油对 DPPH 和 ABTS 自由基具有相当高的自由基清除活性，其 IC 值分别为 70 和 105μg/mL（作为标准的抗坏血酸，其 IC 值分别为 32 和 29μg/mL 针对上述自由基）。精油还表现出抗胆碱酯酶活性，对 AChE 和 BChE 的 IC 值分别为 48 和 90μg/mL（作为标准加兰他敏，其 IC 值分别为 25 和 30μg/mL）。该精油还表现出抗糖尿病潜力，对 α-葡萄糖苷酶和 α-淀粉酶的 IC 值分别为 120 和 110μg/mL（作为标准阿卡波糖的 IC 值分别为 28 和 30μg/mL）。