Massounga Bora Awa Fanny, Li Xiaodong, Liu Lu
College of Food Science, Northeast Agricultural University, No. 59 Mucai St., Xiangfang Dist., Harbin 150030, China.
Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, No. 600 Changjiang St., Xiangfang Dist., Harbin 150030, China.
Foods. 2021 Nov 3;10(11):2677. doi: 10.3390/foods10112677.
The identification of novel sources of synbiotic agents with desirable functionality is an emerging concept. In the present study, novel encapsulates containing probiotic LA-05 (LA) and Charantin (CT) were produced by freeze-drying technique using pure Whey Protein Isolate (WPI), pure Maltodextrin (MD), and their combination (WPI + MD) in 1:1 core ratio, respectively. The obtained microparticles, namely WPI + LA + CT, MD + LA + CT, and WPI + MD + LA + CT were tested for their physicochemical properties. Among all formulations, combined carriers (WPI + MD) exhibited the highest encapsulation yields for LA (98%) and CT (75%). Microparticles showed a mean d (4, 3) ranging from 50.393 ± 1.26 to 68.412 ± 3.22 μm. The Scanning Electron Microscopy revealed uniformly amorphous and glass-like structures, with a noticeably reduced porosity when materials were combined. In addition, Fourier Transform Infrared spectroscopy highlighted the formation of strong hydrogen bonds supporting the interactions between the carrier materials (WPI and MD) and CT. In addition, the thermal stability of the combined WPI + MD was superior to that of pure WPI and pure MD, as depicted by the Thermogravimetric and Differential Scanning Calorimetry analysis. More interestingly, co-encapsulation with CT enhanced LA viability (8.91 ± 0.3 log CFU/g) and Cells Surface Hydrophobicity (82%) in vitro, in a prebiotic-like manner. Correspondingly, CT content was heightened when co-encapsulated with LA. Besides, WPI + MD + LA + CT microparticles exhibited higher antioxidant activity (79%), α-amylase inhibitory activity (83%), and lipase inhibitory activity (68%) than single carrier ones. Furthermore, LA viable count (7.95 ± 0.1 log CFU/g) and CT content (78%) were the highest in the blended carrier materials after 30 days of storage at 4 °C. Synbiotic microparticle WPI + MD + LA + CT represents an effective and promising approach for the co-delivery of probiotic culture and bioactive compounds in the digestive tract, with enhanced functionality and storage properties.
鉴定具有理想功能的新型合生元制剂来源是一个新兴概念。在本研究中，分别使用纯乳清蛋白分离物（WPI）、纯麦芽糊精（MD）及其1:1核心比例的组合（WPI + MD），通过冷冻干燥技术制备了含有益生菌LA-05（LA）和苦瓜素（CT）的新型包囊。对获得的微粒，即WPI + LA + CT、MD + LA + CT和WPI + MD + LA + CT进行了物理化学性质测试。在所有制剂中，复合载体（WPI + MD）对LA（98%）和CT（75%）表现出最高的包封率。微粒的平均d（4, 3）范围为50.393±1.26至68.412±3.22μm。扫描电子显微镜显示为均匀的无定形和玻璃状结构，材料混合时孔隙率明显降低。此外，傅里叶变换红外光谱突出了强氢键的形成，支持载体材料（WPI和MD）与CT之间的相互作用。此外，热重分析和差示扫描量热法分析表明，复合WPI + MD的热稳定性优于纯WPI和纯MD。更有趣的是，与CT共包封以益生元样方式提高了LA在体外的活力（8.91±0.3 log CFU/g）和细胞表面疏水性（82%）。相应地，与LA共包封时CT含量增加。此外，WPI + MD + LA + CT微粒比单一载体微粒表现出更高的抗氧化活性（79%）、α-淀粉酶抑制活性（83%）和脂肪酶抑制活性（68%）。此外，在4℃储存30天后，混合载体材料中的LA活菌数（7.95±0.1 log CFU/g）和CT含量（78%）最高。合生元微粒WPI + MD + LA + CT代表了一种在消化道中共递送益生菌培养物和生物活性化合物的有效且有前景的方法，具有增强的功能和储存特性。
