Zhang Zhidong, Sun Yang, Yi Yuanyang, Bai Xiaoyu, Zhu Liying, Zhu Jing, Gu Meiying, Zhu Yanlei, Jiang Ling
Xinjiang Key Laboratory of Special Environmental Microbiology, Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China.
College of Life Sciences, Xinjiang Normal University, Urumqi 830054, China.
Microorganisms. 2023 Aug 13;11(8):2079. doi: 10.3390/microorganisms11082079.
Quorum-sensing (QS) is involved in numerous physiological processes in bacteria, such as biofilm formation, sporulation, and virulence formation. Therefore, the search for new quorum-sensing inhibitors (QSI) is a promising strategy that opens up a new perspective for controlling QS-mediated bacterial pathogens. To explore new QSIs, a strain named sp. D67 with QS inhibitory activity was isolated from the soil of the arid zone around the Kumutag Desert in Xinjiang. Phylogenetic analyses demonstrated that strain D67 shared the highest similarity with NBRC 15402T (98.39%), which indicated it represented a potential novel species in the genus. The fermentation crude extracts of strain D67 can effectively reduce the violacein production produced by CV026 and the swarming and swimming abilities of . It also has significant inhibitory activity on the production of virulence factors such as biofilm, pyocyanin, and rhamnolipids of in a significant concentration-dependent manner, but not on protease activity. A total of 618 compounds were identified from the fermentation crude extracts of strain D67 by LC-MS, and 19 compounds with significant QS inhibitory activity were observed. Overall, the strain with QS inhibitory activity was screened from Kumutag Desert in Xinjiang for the first time, which provided a basis for further research and development of new QSI.
群体感应（QS）参与细菌的众多生理过程，如生物膜形成、孢子形成和毒力形成。因此，寻找新的群体感应抑制剂（QSI）是一种有前景的策略，为控制QS介导的细菌病原体开辟了新的视角。为了探索新的QSI，从新疆库姆塔格沙漠周边干旱地区的土壤中分离出一株具有QS抑制活性的菌株，命名为sp. D67。系统发育分析表明，菌株D67与NBRC 15402T的相似度最高（98.39%），这表明它代表了该属中的一个潜在新物种。菌株D67的发酵粗提物能有效降低CV026产生的紫色菌素产量以及的群体游动和泳动能力。它还对的生物膜、绿脓菌素和鼠李糖脂等毒力因子的产生具有显著的抑制活性，且呈明显的浓度依赖性，但对蛋白酶活性没有影响。通过LC-MS从菌株D67的发酵粗提物中鉴定出总共618种化合物，观察到19种具有显著QS抑制活性的化合物。总体而言，首次从新疆库姆塔格沙漠筛选出具有QS抑制活性的菌株，为新QSI的进一步研发提供了依据。
