Kesanakurti Prasad, Ragupathy Subramanyam, Faller Adam C, Shanmughanandhan Dhivya, Buongiorno Francesco, Della Noce Isabella, Lu Zhengfei, Zhang Yanjun, Newmaster Steven G
NHP Research Alliance, College of Biological Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
Hyris Ltd., Lower Ground Floor, One George Yard, London EC3V 9DF, UK.
Foods. 2021 Nov 5;10(11):2705. doi: 10.3390/foods10112705.
Authentication of and products is important to be able to mitigate instances of adulteration and substitution that exist within the international supply chain of ginseng. To address this issue, species-specific hydrolysis probe qPCR assays were developed and validated for both and herbal dietary supplements. Performance of the probe-based assays was evaluated using analytical validation criteria, which included evaluation of: (1) specificity, in selectively identifying the target species; (2) sensitivity, in detecting the lowest amount of the target material; and (3) repeatability and reproducibility of the method in detecting the target species in raw materials on a real-time PCR platform (reliability). The species-specific probes were developed and successfully passed the validation criteria with 100% specificity, 80-120% efficiency and 100% reliability. The methods developed in this study are fit for purpose, rapid, and easy to implement in quality assurance programs; authentication of ginseng herbal supplements is possible, even with extracts where DNA is fragmented and of low quality and quantity.
人参及相关产品的鉴定对于减少人参国际供应链中存在的掺假和替代情况至关重要。为解决这一问题，针对人参及相关草药膳食补充剂开发并验证了物种特异性水解探针qPCR检测方法。基于探针的检测方法的性能使用分析验证标准进行评估，其中包括评估：（1）特异性，即选择性识别目标物种；（2）灵敏度，即检测目标物质的最低量；（3）该方法在实时PCR平台上检测原材料中目标物种的重复性和再现性（可靠性）。开发了物种特异性探针，并成功通过验证标准，特异性为100%，效率为80 - 120%，可靠性为100%。本研究中开发的方法符合目的，快速且易于在质量保证计划中实施；即使对于DNA片段化且质量和数量较低的提取物，也能够对人参草药补充剂进行鉴定。
