Wang Rong-Bo, Tian Hui-Li, Wang Hong-Tao, Li Gui-Sheng
School of Pharmacy, Yantai University, Yantai 264005, China.
College of Life Science, Yantai University, Yantai 264005, China.
Zhongguo Zhong Yao Za Zhi. 2018 Apr;43(7):1441-1445. doi: 10.19540/j.cnki.cjcmm.20180201.004.
Panax ginseng and P. quinquefolius are two kinds of important medicinal herbs. They are morphologically similar but have different pharmacological effects. Therefore, botanical origin authentication of these two ginsengs is of great importance for ensuring pharmaceutical efficacy and food safety. Based on the fact that intron position in orthologous genes is highly conserved across plant species, intron length polymorphisms were exploited from unigenes of ginseng. Specific primers were respectively designed for these two species based on their insertion/deletion sequences of cytochrome P450 and glyceraldehyde 3-phosphate dehydrogenase, and multiplex PCR was conducted for molecular authentication of P.ginseng and P. quinquefolius. The results showed that the developed multiplex PCR assay was effective for molecular authentication of P.ginseng and P. quinquefolius without strict PCR condition and the optimization of reaction system.This study provides a preferred ideal marker system for molecular authentication of ginseng,and the presented method can be employed in origin authentication of other herbal preparations.
人参和西洋参是两种重要的药用植物。它们形态相似但药理作用不同。因此，对这两种人参进行植物来源鉴定对于确保药效和食品安全至关重要。基于直系同源基因的内含子位置在不同植物物种间高度保守这一事实，从人参单基因中开发了内含子长度多态性。根据细胞色素P450和甘油醛-3-磷酸脱氢酶的插入/缺失序列分别为这两个物种设计了特异性引物，并进行多重PCR以对人参和西洋参进行分子鉴定。结果表明，所建立的多重PCR检测方法在不严格的PCR条件和无需优化反应体系的情况下，对人参和西洋参的分子鉴定有效。本研究为人参的分子鉴定提供了一种优选的理想标记系统，所提出的方法可用于其他草药制剂的来源鉴定。
