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黑荆及其活性成分可减轻城市颗粒物诱导的眼表损伤后的氧化应激、炎症和细胞凋亡。

Thunberg and Its Active Components Mitigate Oxidative Stress, Inflammation and Apoptosis after Urban Particulate Matter-Induced Ocular Surface Damage.

作者信息

Kang Wan Seok, Choi Hakjoon, Lee Ki Hoon, Kim Eun, Kim Kyeong Jo, Kim Jin Seok, Na Chang-Su, Kim Sunoh

机构信息

Central R&D Center, B&Tech Co., Ltd., Gwangju 61239, Korea.

College of Korean Medicine, Dongshin University, Naju-si 58245, Korea.

出版信息

Antioxidants (Basel). 2021 Oct 28;10(11):1717. doi: 10.3390/antiox10111717.

DOI:10.3390/antiox10111717
PMID:34829588
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8614870/
Abstract

We previously demonstrated that urban particulate matter (UPM) exposure decreases the migration activity and survival of human corneal epithelial cells (HCECs). Herein, we investigated the potential to improve the corneal wound-healing ability of Thunb. leaf extract (PJE) and its active components on UPM-induced ocular surface damage in vitro and in vivo. PJE effectively assisted wound healing without altering HCEC survival and enhanced catalase (CAT), heme oxygenase 1 (HO1) and glutathione peroxidase 1 (GPX1) antioxidant gene expression. A corneal wound was uniformly induced on the right eye in all experimental animals and divided into eight groups such as two control groups (wounded right eye group-NR and non-wounded left eye group-NL), UPM treated group and PJEs (25, 50, 100, 200, 400 mg/kg) treated groups. Corneal abrasion model rats exposed to UPM showed delayed wound healing compared to unexposed rats, but wound healing was dose-dependently enhanced by PJE oral administration. Seventy-two hours after wound generation, inflammatory cells, apoptotic cells and interleukin-6 (IL-6) expression were increased substantially after UPM exposure, but PJE treatment significantly reduced the wound to an almost normal level while enhancing re-epithelialization without changing corneal thickness. Next, we tried to identify the key molecules for enhancing wound healing through fractionation. The major compounds in the fraction, confirmed by high-performance liquid chromatography (HPLC), were chlorogenic acid (CA), neochlorogenic acid (NCA) and cryptochlorogenic acid (CCA). Each type of CA isomers showed slightly different half maximal effective (EC50) and maximal effective (ECmax) concentrations, and their mixtures synergistically enhanced HCEC migration. Thus, corneal abrasion wound recovery after UPM exposure improved after PJE treatment, and the active PJE components were identified, providing an important basis to develop therapeutics for ocular surface damage using PJE.

摘要

我们之前证明，暴露于城市颗粒物（UPM）会降低人角膜上皮细胞（HCEC）的迁移活性和存活率。在此，我们研究了番泻叶提取物（PJE）及其活性成分改善角膜伤口愈合能力的潜力，以及其对体外和体内UPM诱导的眼表损伤的作用。PJE有效促进伤口愈合，且不改变HCEC存活率，并增强了过氧化氢酶（CAT）、血红素加氧酶1（HO1）和谷胱甘肽过氧化物酶1（GPX1）抗氧化基因的表达。在所有实验动物的右眼均匀诱导角膜伤口，并分为八组，如两个对照组（受伤右眼组-NR和未受伤左眼组-NL）、UPM处理组和PJE（25、50、100、200、400mg/kg）处理组。与未暴露于UPM的大鼠相比，暴露于UPM的角膜擦伤模型大鼠伤口愈合延迟，但口服PJE可剂量依赖性地增强伤口愈合。伤口产生72小时后，UPM暴露后炎症细胞、凋亡细胞和白细胞介素-6（IL-6）表达显著增加，但PJE处理显著将伤口减少至几乎正常水平，同时增强再上皮化，而不改变角膜厚度。接下来，我们试图通过分级分离鉴定增强伤口愈合的关键分子。通过高效液相色谱（HPLC）确认的分级分离中的主要化合物为绿原酸（CA）、新绿原酸（NCA）和隐绿原酸（CCA）。每种类型的CA异构体显示出略有不同的半数最大效应（EC50）和最大效应（ECmax）浓度，并且它们的混合物协同增强HCEC迁移。因此，PJE处理后，UPM暴露后的角膜擦伤伤口恢复得到改善，并且鉴定出了PJE的活性成分，为开发使用PJE治疗眼表损伤的疗法提供了重要依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d673/8614870/8476e99a1986/antioxidants-10-01717-g011.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d673/8614870/8476e99a1986/antioxidants-10-01717-g011.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d673/8614870/898100fa1339/antioxidants-10-01717-g008.jpg
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