Japjec Mladen, Horvat Pavlov Katarina, Petrovic Andreja, Staresinic Mario, Sebecic Bozidar, Buljan Matko, Vranes Hrvoje, Giljanovic Ana, Drmic Domagoj, Japjec Miroslav, Prtoric Andreja, Lovric Eva, Batelja Vuletic Lovorka, Dobric Ivan, Boban Blagaic Alenka, Skrtic Anita, Seiwerth Sven, Predrag Sikiric
Department of Surgery, School of Medicine, University of Zagreb, 10000 Zagreb, Croatia.
Department of Pathology, School of Medicine, University of Zagreb, P.O. Box 910, Salata 10, 10000 Zagreb, Croatia.
Biomedicines. 2021 Oct 27;9(11):1547. doi: 10.3390/biomedicines9111547.
(1) Aim: The stable gastric pentadecapeptide BPC 157 is known to heal transected muscle, tendon, and ligament. Thereby, in this study, we investigated the effect of BPC 157 on the dissection of the quadriceps tendon from the quadriceps muscle in rats. (2) Materials and Methods: Myotendinous junction defect, which cannot heal spontaneously in rats, as evidenced with consistent macro/microscopic, biomechanical, functional assessments, , and mRNA levels and oxidative stress and NO-levels in the myotendinous junctions. BPC 157 (10 µg/kg, 10 ng/kg) regimen was given (i) intraperitoneally, first application immediately after surgery, last 24 h before sacrifice; (ii) per-orally, in drinking water (0.16 µg/mL, 0.16 ng/mL, 12 mL/rat/day), till the sacrifice at 7, 14, 28 and 42 postoperative days. (3) Results: These BPC 157 regimens document prominent therapy effects (macro/microscopic, biomechanical, functional much like and mRNA levels and counteracted oxidative stress and NO-levels in the myotendinous junctions), while controls have a poor presentation. Especially, in rats with the disabled myotendinous junction, along with full functional recovery, BPC 157 counteracts muscle atrophy that is regularly progressive and brings muscle presentation close to normal. Accordingly, unlike the perilous course in controls, those rats, when receiving BPC 157 therapy, exhibit a smaller defect, and finally defects completely disappear. Microscopically, there are no more inflammatory infiltrate, well-oriented recovered tissue of musculotendon junction appears in BPC 157 treated rats at the 28 days and 42 days. (4) Conclusions: BPC 157 restores myotendinous junction in accordance with the healing of the transected muscle, tendon, and ligament.
(1) 目的：已知稳定的胃十五肽BPC 157可促进横断的肌肉、肌腱和韧带愈合。因此，在本研究中，我们调查了BPC 157对大鼠股四头肌肌腱与股四头肌分离的影响。(2) 材料与方法：肌腱结合部缺损在大鼠中无法自发愈合，这已通过一致的宏观/微观、生物力学、功能评估以及肌腱结合部的 、 和mRNA水平以及氧化应激和一氧化氮水平得到证实。给予BPC 157（10μg/kg，10ng/kg）方案：(i) 腹腔注射，术后立即首次给药，在处死前24小时最后一次给药；(ii) 经口给药，加入饮用水中（0.16μg/mL，0.16ng/mL，12mL/大鼠/天），直至术后第7、14、28和42天处死。(3) 结果：这些BPC 157方案显示出显著的治疗效果（宏观/微观、生物力学、功能方面与 、 和mRNA水平相似，并抵消了肌腱结合部的氧化应激和一氧化氮水平），而对照组表现较差。特别是，在肌腱结合部功能受损的大鼠中，随着功能完全恢复，BPC 157抵消了通常会进展的肌肉萎缩，并使肌肉表现接近正常。因此，与对照组的危险病程不同，接受BPC 157治疗的大鼠缺损较小，最终缺损完全消失。在显微镜下，在第28天和第42天，接受BPC 157治疗的大鼠中不再有炎性浸润，出现了排列良好的肌腱结合部恢复组织。(4) 结论：BPC 157可促进肌腱结合部恢复，与横断的肌肉、肌腱和韧带的愈合一致。
