Wu Cheng-Tien, Chen Man-Chih, Liu Shing-Hwa, Yang Ting-Hua, Long Lin-Hwa, Guan Siao-Syun, Chen Chang-Mu
Department of Nutrition, China Medical University, Taichung 406040, Taiwan.
Master Program for Food and Drug Safety, China Medical University, Taichung 406040, Taiwan.
Biomedicines. 2021 Nov 19;9(11):1719. doi: 10.3390/biomedicines9111719.
Stroke, which is the second leading cause of mortality in the world, is urgently needed to explore the medical strategies for ischemic stroke treatment. Both icariin (ICA) and icaritin (ICT) are the major active flavonoids extracted from that have been regarded as the neuroprotective agents in disease models. In this study, we aimed to investigate and compare the neuroprotective effects of ICA and ICT in a middle cerebral artery occlusion (MCAO) mouse model. Male ICR mice were pretreated with both ICA and ICT, which ameliorated body weight loss, neurological injury, infarct volume, and pathological change in acute ischemic stroke mice. Furthermore, administration of both ICA and ICT could also protect against neuronal cell apoptotic death, oxidative and nitrosative stress, lipid peroxidation, and extracellular matrix (ECM) accumulation in the brains. The neuroprotective effects of ICT are slightly better than that of ICA in acute cerebral ischemic stroke mice. These results suggest that pretreatment with both ICA and ICT improves the neuronal cell apoptosis and responses of oxidative/nitrosative stress and counteracts the ECM accumulation in the brains of acute cerebral ischemic stroke mice. Both ICA and ICT treatment may serve as a useful therapeutic strategy for acute ischemic stroke.
中风是全球第二大致死原因，因此迫切需要探索治疗缺血性中风的医学策略。淫羊藿苷（ICA）和淫羊藿次苷（ICT）都是从淫羊藿中提取的主要活性黄酮类化合物，在疾病模型中被视为神经保护剂。在本研究中，我们旨在研究和比较ICA和ICT在大脑中动脉闭塞（MCAO）小鼠模型中的神经保护作用。雄性ICR小鼠用ICA和ICT进行预处理，这改善了急性缺血性中风小鼠的体重减轻、神经损伤、梗死体积和病理变化。此外，给予ICA和ICT还可以防止神经元细胞凋亡死亡、氧化和亚硝化应激、脂质过氧化以及大脑中细胞外基质（ECM）的积累。在急性脑缺血性中风小鼠中，ICT的神经保护作用略优于ICA。这些结果表明，ICA和ICT预处理均可改善神经元细胞凋亡以及氧化/亚硝化应激反应，并对抗急性脑缺血性中风小鼠大脑中的ECM积累。ICA和ICT治疗均可作为急性缺血性中风的有效治疗策略。
