College of Life Science, Kyung Hee University, 1732, Deogyeong-daero, Giheung-gu, Yongin-si, Gyeonggi-do 17104, Republic of Korea.
Photochem Photobiol Sci. 2018 Oct 10;17(10):1396-1408. doi: 10.1039/c8pp00174j.
Icariin (ICA) and icaritin (ICT) exhibit many pharmacological functions including anti-osteoporosis, anti-cardiovascular, and anti-cancer activities; however, there are few comprehensive studies that track the detailed effects on UVB-induced photoaging. The recovery effects of ICA and ICT were investigated in UVB-irradiated human keratinocytes (HaCaTs). The results indicated that ICT and ICA showed strong radical scavenging activity, and the reactive oxygen species (ROS) scavenging activity of ICT was superior. UVB-induced matrix metalloproteinase-1 (MMP-1) expression was blocked by ICA via the inhibition of mitogen-activated protein kinase/activator protein 1 (MAPK/AP-1), which directly reduced extracellular matrix (ECM) degradation. ICT activated nuclear factor erythroid 2 related factor 2 (Nrf2) to improve the anti-oxidative stress capacity and suppress nuclear factor-κB (NF-κB) activation, decreasing vascular endothelial growth factor (VEGF) protein, and inflammatory cytokines induced ECM degrading enzyme secretion. Moreover, ICT was more advantageous to improve transforming growth factor beta 1 (TGF-β1) and procollagen type I expression than ICA, promoting the synthesis of collagen. Therefore, ICA and ICT have potential to treat UVB-induced oxidative stress, inflammation and photoaging, and will be posited as a novel strategy to alleviate photodamage.
朝藿定（ICA）和朝藿定苷（ICT）具有多种药理作用，包括抗骨质疏松、抗心血管和抗癌作用；然而，很少有全面的研究跟踪其对 UVB 诱导的光老化的详细影响。本文研究了 ICA 和 ICT 对 UVB 照射的人角质形成细胞（HaCaTs）的恢复作用。结果表明，ICT 和 ICA 表现出很强的自由基清除活性，并且 ICT 的活性氧（ROS）清除活性更优。ICA 通过抑制丝裂原活化蛋白激酶/激活蛋白 1（MAPK/AP-1）阻断 UVB 诱导的基质金属蛋白酶-1（MMP-1）表达，直接减少细胞外基质（ECM）降解。ICT 通过激活核因子红细胞 2 相关因子 2（Nrf2）来提高抗氧化应激能力并抑制核因子-κB（NF-κB）的激活，减少血管内皮生长因子（VEGF）蛋白和炎症细胞因子诱导的 ECM 降解酶分泌。此外，ICT 比 ICA 更有利于提高转化生长因子 β1（TGF-β1）和 I 型前胶原的表达，促进胶原的合成。因此，ICA 和 ICT 具有治疗 UVB 诱导的氧化应激、炎症和光老化的潜力，并将被提出作为缓解光损伤的一种新策略。
