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兔脂肪组织来源间充质干细胞的表型特征和神经分化。

Phenotypical Characterization and Neurogenic Differentiation of Rabbit Adipose Tissue-Derived Mesenchymal Stem Cells.

机构信息

AgroBioTech Research Center, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia.

Department of Biochemistry and Biotechnology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia.

出版信息

Genes (Basel). 2021 Mar 17;12(3):431. doi: 10.3390/genes12030431.

DOI:10.3390/genes12030431
PMID:33802902
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8002684/
Abstract

Although the rabbit is a frequently used biological model, the phenotype of rabbit adipose-derived mesenchymal stem cells (rAT-MSCs) is not well characterized. One of the reasons is the absence of specific anti-rabbit antibodies. The study aimed to characterize rAT-MSCs using flow cytometry and PCR methods, especially digital droplet PCR, which confirmed the expression of selected markers at the mRNA level. A combination of these methods validated the expression of MSCs markers (CD29, CD44, CD73, CD90 and CD105). In addition, cells were also positive for CD49f, vimentin, desmin, α-SMA, ALDH and also for the pluripotent markers: NANOG, OCT4 and SOX2. Moreover, the present study proved the ability of rAT-MSCs to differentiate into a neurogenic lineage based on the confirmed expression of neuronal markers ENO2 and MAP2. Obtained results suggest that rAT-MSCs have, despite the slight differences in marker expression, the similar phenotype as human AT-MSCs and possess the neurodifferentiation ability. Accordingly, rAT-MSCs should be subjected to further studies with potential application in veterinary medicine but also, in case of their cryopreservation, as a source of genetic information of endangered species stored in the gene bank.

摘要

虽然兔子是一种常用的生物模型，但兔脂肪间充质干细胞（rAT-MSCs）的表型尚未得到很好的描述。原因之一是缺乏特异性的抗兔抗体。本研究旨在使用流式细胞术和 PCR 方法，特别是数字液滴 PCR，来鉴定 rAT-MSCs，以确认所选标记物在 mRNA 水平的表达。这些方法的组合验证了间充质干细胞标志物（CD29、CD44、CD73、CD90 和 CD105）的表达。此外，细胞还对 CD49f、波形蛋白、结蛋白、α-SMA、ALDH 以及多能性标记物：NANOG、OCT4 和 SOX2 呈阳性。此外，本研究还证明了 rAT-MSCs 基于神经元标记物 ENO2 和 MAP2 的表达具有向神经谱系分化的能力。这些结果表明，尽管 rAT-MSCs 标志物的表达存在细微差异，但它们具有与人 AT-MSCs 相似的表型，并具有神经分化能力。因此，rAT-MSCs 应该进一步研究，以便在兽医医学中有潜在的应用，并且在冷冻保存的情况下，也可以作为基因库中濒危物种遗传信息的来源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8eb/8002684/431949955e5f/genes-12-00431-g009.jpg
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