State Key Laboratory for Managing Biotic and Chemical Threats to The Quality and Safety of Agro-Products, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
State Key Lab of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing 210095, China.
Int J Mol Sci. 2021 Nov 15;22(22):12317. doi: 10.3390/ijms222212317.
GDSL-type esterase/lipase proteins (GELPs) characterized by a conserved GDSL motif at their N-terminus belong to the lipid hydrolysis enzyme superfamily. In plants, GELPs play an important role in plant growth, development and stress response. The studies of the identification and characterization of the gene family in Triticeae have not been reported. In this study, 193 were identified in and classified into 11 groups (clade A-K) by means of phylogenetic analysis. Most contain only one GDSL domain, only four contain other domains besides the GDSL domain. Gene structure analysis indicated 35.2% genes have four introns and five exons. In the promoter regions of the identified , we detected 4502 putative -elements, which were associated with plant hormones, plant growth, environmental stress and light responsiveness. Expression profiling revealed 36, 44 and 17 were highly expressed in the spike, the root and the grain, respectively. Further investigation of a root-specific expressing , , indicated it was induced by a variety of biotic and abiotic stresses. The knockdown of inhibited long-distance movement of BSMV in the tissue of . This research provides a genome-wide glimpse of the genes and hints at the participation of in the interaction between virus and plants.
GDSL 型酯酶/脂肪酶蛋白（GELP）在其 N 端具有保守的 GDSL 基序，属于脂质水解酶超家族。在植物中，GELP 在植物生长、发育和应激反应中发挥重要作用。关于禾本科植物中 GELP 基因家族的鉴定和特征研究尚未见报道。本研究在中鉴定了 193 个，并通过系统发育分析将其分为 11 组（分支 A-K）。大多数 只包含一个 GDSL 结构域，只有四个 除了 GDSL 结构域外还包含其他结构域。基因结构分析表明，35.2%的 基因具有四个内含子和五个外显子。在所鉴定的 启动子区域中，我们检测到 4502 个假定的 - 元件，这些元件与植物激素、植物生长、环境胁迫和光响应有关。表达谱分析显示，36、44 和 17 个 在穗、根和籽粒中分别高度表达。对一个根特异性表达的 进一步研究表明，它受到多种生物和非生物胁迫的诱导。 的敲低抑制了 BSMV 在 的组织中的长距离运动。本研究提供了 基因的全基因组视角，并暗示 参与了病毒与植物之间的相互作用。
