Admas Tayachew, Wudu Maru, Berhanie Hailu
Research Center of Crop Stresses Resistance Technologies/ MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River, Yangtze University, Jingzhou, 434025, China.
Department of Biology, Debark University, Debark, Ethiopia.
Funct Integr Genomics. 2024 Dec 27;25(1):2. doi: 10.1007/s10142-024-01508-7.
The barley stripe mosaic virus (BSMV) uses its genomic RNA components (alpha, beta, and gamma) as an efficient method for studying gene functions. It is a newly developed method that utilizes gene transcript suppression to determine the role of plant genes. BSMV derived from virus induced gene silencing (VIGS) is capable of infecting various key farming crops like barley, wheat, rice, corn, and oats. Nevertheless, the growing acceptance and enhancement of BSMV-VIGS will benefit all kinds of plants. Abiotic stresses such as drought and salt are highly affecting plant growth, development, and production. BSMV-induced temporal gene knockdown is performed during particular stressful situations to determine their specific function. The quick physiological and biochemical changes aid in confirming the role of the target genes. VIGS has a significant role to improve crop genetics and breeding, despite having certain restrictions. Thus, exploring the possible solution and addressing these difficulties will enhance the technology in the continuous advancement of plant manufacturing. BSMV-mediated VIGS has become popular in functional genomics; gene function can be determined without permanent transformation. In general, BSMV-mediated VIGS will be very helpful in the ongoing effort to develop resilient crops.
大麦条纹花叶病毒（BSMV）利用其基因组RNA组分（α、β和γ）作为研究基因功能的有效方法。这是一种新开发的利用基因转录抑制来确定植物基因作用的方法。源自病毒诱导基因沉默（VIGS）的BSMV能够感染各种主要农作物，如大麦、小麦、水稻、玉米和燕麦。然而，BSMV-VIGS越来越被接受并得到改进，这将惠及各类植物。干旱和盐等非生物胁迫对植物的生长、发育和产量影响很大。在特定的胁迫条件下进行BSMV诱导的瞬时基因敲低，以确定其特定功能。快速的生理和生化变化有助于确认靶基因的作用。尽管存在某些限制，但VIGS在改善作物遗传学和育种方面具有重要作用。因此，探索可能的解决方案并解决这些困难将在植物生产的持续进步中提升这项技术。BSMV介导的VIGS在功能基因组学中已变得很流行；无需进行永久转化就能确定基因功能。总的来说，BSMV介导的VIGS将对当前培育抗逆作物的努力非常有帮助。
