Environmental Research and Innovation Department, Luxembourg Institute of Science and Technology, 5, Rue Bommel, Hautcharage, L-4940 Luxembourg, Luxembourg.
Int J Mol Sci. 2021 Nov 15;22(22):12319. doi: 10.3390/ijms222212319.
Callogenesis, the process during which explants derived from differentiated plant tissues are subjected to a trans-differentiation step characterized by the proliferation of a mass of cells, is fundamental to indirect organogenesis and the establishment of cell suspension cultures. Therefore, understanding how callogenesis takes place is helpful to plant tissue culture, as well as to plant biotechnology and bioprocess engineering. The common herbaceous plant stinging nettle ( L.) is a species producing cellulosic fibres (the bast fibres) and a whole array of phytochemicals for pharmacological, nutraceutical and cosmeceutical use. Thus, it is of interest as a potential multi-purpose plant. In this study, callogenesis in internode explants of a nettle fibre clone (clone 13) was studied using RNA-Seq to understand which gene ontologies predominate at different time points. Callogenesis was induced with the plant growth regulators α-napthaleneacetic acid (NAA) and 6-benzyl aminopurine (BAP) after having determined their optimal concentrations. The process was studied over a period of 34 days, a time point at which a well-visible callus mass developed on the explants. The bioinformatic analysis of the transcriptomic dataset revealed specific gene ontologies characterizing each of the four time points investigated (0, 1, 10 and 34 days). The results show that, while the advanced stage of callogenesis is characterized by the iron deficiency response triggered by the high levels of reactive oxygen species accumulated by the proliferating cell mass, the intermediate and early phases are dominated by ontologies related to the immune response and cell wall loosening, respectively.
愈伤组织发生，是指外植体经历一个转分化步骤的过程，其特征是大量细胞的增殖，这是间接器官发生和细胞悬浮培养建立的基础。因此，了解愈伤组织发生的过程有助于植物组织培养，以及植物生物技术和生物工艺工程。常见的草本荨麻（L.）是一种产生纤维素纤维（韧皮纤维）和一系列植物化学物质的物种，可用于药理学、营养保健品和化妆品。因此，它作为一种潜在的多用途植物很有意义。在这项研究中，使用 RNA-Seq 研究了荨麻纤维克隆（克隆 13）的节间外植体的愈伤组织发生，以了解在不同时间点哪些基因本体论占主导地位。在确定了最佳浓度后，用植物生长调节剂α-萘乙酸（NAA）和 6-苄基氨基嘌呤（BAP）诱导愈伤组织发生。该过程研究了 34 天，此时外植体上形成了可见的愈伤组织块。转录组数据集的生物信息学分析揭示了每个研究的四个时间点（0、1、10 和 34 天）的特定基因本体论。结果表明，虽然愈伤组织发生的高级阶段的特征是由增殖细胞团积累的高水平活性氧引起的缺铁反应，但中间和早期阶段分别由与免疫反应和细胞壁松弛相关的本体论主导。
