Laboratory of Applied Genomics and Crop Breeding, All-Russia Research Institute of Agricultural Biotechnology, 127550 Moscow, Russia.
Kurchatov Genomics Center-ARRIAB, All-Russia Research Institute of Agricultural Biotechnology, 127550 Moscow, Russia.
Int J Mol Sci. 2021 Nov 16;22(22):12376. doi: 10.3390/ijms222212376.
The Growth-regulating factors (GRF) are a family of plant-specific transcription factors that have roles in plant growth, development and stress response. In this study the diversity of the (TraesCS2A02G435100) gene was investigated in Russian bread wheat germplasm by means of next generation sequencing and molecular markers, and the results compared with those from multiple wheat genome and exome sequencing projects. The results showed that an allele possessing c.495G>T polymorphism found in Bezostaya 1 and designated as , is connected with earlier heading and better grain filling under conditions of the Krasnodar Krai. is more frequent among Russian winter wheat cultivars than in other germplasms found in the world, implying that it is adaptive for the Chernozem region. A new rare mutation of the was found in the spring wheat cultivar Novosibirskaya 67. The molecular markers developed will facilitate utilization of mutations in future agronomic studies and wheat improvement. Albeit may be good at maintaining high milling quality of the grain, it should be used with caution in breeding of winter wheat cultivars in the perspective of climate change.
生长调节因子（GRF）是一类植物特异性转录因子，在植物生长、发育和应激反应中发挥作用。本研究采用下一代测序和分子标记技术，对俄罗斯面包小麦种质资源中的（TraesCS2A02G435100）基因进行了多样性研究，并与多个小麦基因组和外显子测序项目的结果进行了比较。结果表明，在 Bezostaya 1 中发现的具有 c.495G>T 多态性的等位基因，被指定为 ，与克拉斯诺达尔边疆区条件下的早期抽穗和更好的灌浆有关。在俄罗斯冬小麦品种中比在世界其他种质资源中更为常见，表明它适应黑土地区。在春小麦品种 Novosibirskaya 67 中发现了 一个新的罕见突变。开发的分子标记将有助于在未来的农业研究和小麦改良中利用 突变。尽管 可能擅长保持谷物的高出粉率，但在考虑气候变化的情况下，在冬小麦品种的选育中应谨慎使用。
