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斜带石斑鱼四个nanos基因的差异表达模式及其功能意义

Divergent Expression Patterns and Function Implications of Four nanos Genes in a Hermaphroditic Fish, Epinephelus coioides.

作者信息

Sun Zhi-Hui, Wang Yang, Lu Wei-Jia, Li Zhi, Liu Xiao-Chun, Li Shui-Sheng, Zhou Li, Gui Jian-Fang

机构信息

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate University of the Chinese Academy of Sciences, Wuhan 430072, China.

State Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Aquatic Economic Animals, The Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.

出版信息

Int J Mol Sci. 2017 Mar 23;18(4):685. doi: 10.3390/ijms18040685.

DOI:10.3390/ijms18040685
PMID:28333083
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5412271/
Abstract

Multiple genes have been characterized in several fishes, but the functional implications of their various expression patterns remain unclear. In this study, we identified and characterized four genes from a hermaphroditic fish orange-spotted grouper, . and show divergent expression patterns, and the dynamic expression change of in pituitaries during sex change is associated with testis differentiation and spermatogenesis. and might be germline stem cells (GSCs) and primordial germ cells (PGCs)-specific markers, respectively. Significantly, 3'-untranslated region (UTR) is necessary for PGC specific expression, where a non-canonical "GCACGTTT" sequence is required for miR-430-mediated repression of RNA. Furthermore, grouper Dead end (Dnd) can relieve miR-430 repression in PGCs by associating with a 23 bp U-rich region (URR) in 3'-UTR. The current study revealed the functional association of multiple genes with PGC formation and germ cell development in orange-spotted grouper, and opened up new possibilities for developing biotechnologies through utilizing the associations between and PGCs or between and GSCs in the hermaphroditic fish.

摘要

多个基因已在几种鱼类中得到表征，但其各种表达模式的功能意义仍不清楚。在本研究中，我们从雌雄同体的鱼类点带石斑鱼中鉴定并表征了四个基因。[基因名称1]和[基因名称2]表现出不同的表达模式，并且[基因名称3]在性别转换期间垂体中的动态表达变化与睾丸分化和精子发生有关。[基因名称4]和[基因名称5]可能分别是生殖系干细胞（GSCs）和原始生殖细胞（PGCs）的特异性标记。值得注意的是，[基因名称6]的3'非翻译区（UTR）对于PGC特异性表达是必需的，其中非经典的“GCACGTTT”序列是miR-430介导的[基因名称6]RNA抑制所必需的。此外，石斑鱼Dead end（Dnd）可以通过与[基因名称6] 3'-UTR中的一个23 bp富含U的区域（URR）结合来减轻PGCs中miR-430的抑制作用。目前的研究揭示了多个[基因名称]基因与点带石斑鱼PGC形成和生殖细胞发育之间的功能关联，并通过利用雌雄同体鱼类中[基因名称]与PGCs之间或[基因名称]与GSCs之间的关联为开发生物技术开辟了新的可能性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2aef/5412271/6423c427da65/ijms-18-00685-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2aef/5412271/773607305799/ijms-18-00685-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2aef/5412271/8d7b070eac4e/ijms-18-00685-g002.jpg
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