Yan Jichen, Xing Zhifu, Lei Piao, Sikandar Aatika, Yang Ruowei, Wang Yuanyuan, Zhu Xiaofeng, Liu Xiaoyu, Fan Haiyan, Xuan Yuanhu, Chen Lijie, Duan Yuxi
Nematology Institute of Northern China, Shenyang Agricultural University, Shenyang 110866, China.
College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China.
Life (Basel). 2021 Oct 26;11(11):1143. doi: 10.3390/life11111143.
Soybean cyst nematode (SCN) ( Ichinohe) is responsible for causing a major soybean disease globally. The fungal strain Snef1650 was evaluated against . However, the effective determinants of the strain are unknown. By performing pot experiments, a functioning compound was isolated from Snef1650 through organic solvent extraction, semi-preparative HPLC, Sephadex LH-20 column chromatography, and silica gel column chromatography, and the isolated compound was identified to be scopoletin through 1H NMR, 13C NMR, and HPLC-MS. The pot experiments indicated that the treatment of soybean seeds with scopoletin drastically reduced the SCN population. The field experiments performed in 2017 and 2018 revealed that scopoletin decreased over 43.7% juveniles in the roots and over 61.55% cysts in the soil. Scopoletin treatment also promoted soybean growth and improved its yield, with an increase in plot yield by >5.33%. Scopoletin obtained from Snef1650 could be used as an anti- biocontrol agent.
大豆胞囊线虫(SCN)(稻野)是全球范围内导致大豆发生重大病害的病原体。对真菌菌株Snef1650进行了抗大豆胞囊线虫的评估。然而,该菌株的有效决定因素尚不清楚。通过盆栽实验,通过有机溶剂萃取、半制备高效液相色谱、葡聚糖凝胶LH - 20柱色谱和硅胶柱色谱从Snef1650中分离出一种起作用的化合物,并通过1H NMR、13C NMR和HPLC - MS鉴定该分离出的化合物为东莨菪素。盆栽实验表明,用东莨菪素处理大豆种子可大幅减少大豆胞囊线虫的数量。2017年和2018年进行的田间实验表明,东莨菪素使根部的幼虫数量减少了43.7%以上,土壤中的胞囊数量减少了61.55%以上。东莨菪素处理还促进了大豆生长并提高了产量,小区产量增加超过5.33%。从Snef1650中获得的东莨菪素可作为一种生物防治剂。