Department of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-718 Olsztyn, Poland.
Department of Environmental Sciences, Vytautas Magnus University, Universiteto 10, 46265 Kaunas, Lithuania.
Molecules. 2021 Nov 9;26(22):6765. doi: 10.3390/molecules26226765.
In this study, the ability of L. to recover to normal growth, after being degraded in a tetracycline-containing medium, was extensively investigated. The plants were exposed to tetracycline (TC) at concentrations of 1, 2.5, and 10 mM. Subsequently, their physiological status was analysed against the following criteria: rate of plant growth; free radical accumulation; antioxidant enzyme activity; chlorophyll content; HSP70 protein content; cell membrane permeability, and mitochondrial activity. The study showed that duckweed can considerably recover from the damage caused by antibiotics, within a week of cessation of stress. Of the plant properties analysed, mitochondrial activity was the most sensitive to antibiotic-induced disturbances. After transferring the plants to a tetracycline-free medium, all plant parameters improved significantly, except for the mitochondrial activity in the plants grown on the medium containing the highest dose of tetracycline. In the plants treated with this antibiotic at the concentration of 10 mM, the proportion of dead mitochondria increased and was as high as 93% after one week from the beginning of the recovery phase, even after the transfer to the tetracycline-free medium.
在这项研究中，广泛研究了 L. 在含有四环素的培养基中降解后恢复正常生长的能力。将植物暴露于浓度为 1、2.5 和 10 mM 的四环素中。随后，根据以下标准分析它们的生理状态：植物生长速度；自由基积累；抗氧化酶活性；叶绿素含量；HSP70 蛋白含量；细胞膜通透性和线粒体活性。研究表明，浮萍在停止应激后的一周内，可以从抗生素引起的损伤中得到显著恢复。在所分析的植物特性中，线粒体活性对抗生素引起的干扰最为敏感。将植物转移到不含四环素的培养基后，除了在含有最高剂量四环素的培养基中生长的植物的线粒体活性外，所有植物参数都显著改善。在浓度为 10 mM 的抗生素处理的植物中，死亡的线粒体比例增加，在恢复阶段开始后一周内甚至转移到不含四环素的培养基中时，高达 93%。
