Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, 14 Św. Marii Magdaleny, 61-861 Poznań, Poland.
Institute of Plant Genetics, Polish Academy of Sciences, 34 Strzeszyńska St., 60-479 Poznań, Poland.
Molecules. 2021 Nov 11;26(22):6823. doi: 10.3390/molecules26226823.
L. (Twinflower)-a dwarf shrub in the Linnaeeae tribe of Caprifoliaceae family-is distributed across the Northern Hemisphere. By means of this study, a reliable protocol for efficient micropropagation of uniform L. var. plantlets has been provided for the first time; callus culture was also established. Different initial explants, types of cultures, media systems, and plant growth regulators in Murashige and Skoog (MS) media were tested. Agitated shoot cultures in the liquid media turned out to be the best system for the production of sustainable plant biomass. After stabilization of the callus lines, the highest growth index (c.a. 526%) was gained for callus maintained on MS enriched with picloram. TLC and UHPLC-HESI-HRMS analysis confirmed the presence of phenolic acids and flavonoids, and for the first time, the presence of iridoids and triterpenoid saponins in this species. Multiplication of shoot culture provides renewable raw material, allowing for the assessment of the phytochemical profile, and, in the future, for the quantitative analyses and the studies of the biological activity of extracts, fractions, or isolated compounds. This is the first report on in vitro cultures of traditionally used rare taxon and its biosynthetic potential.
L.（双花木）-玄参科忍冬族的矮灌木-分布于北半球。本研究首次为均匀 L. var. 植物小植株的高效微繁殖提供了可靠的方案；还建立了愈伤组织培养。在 Murashige 和 Skoog (MS) 培养基中测试了不同的初始外植体、培养类型、培养基系统和植物生长调节剂。结果表明，在液体培养基中振荡的芽培养是生产可持续植物生物量的最佳系统。在稳定愈伤组织系后，在用 picloram 富集的 MS 培养基上维持的愈伤组织获得了最高的生长指数（约 526%）。TLC 和 UHPLC-HESI-HRMS 分析证实了酚酸和类黄酮的存在，并且首次在该物种中存在环烯醚萜和三萜皂苷。芽培养的繁殖提供了可再生的原料，可用于评估植物化学特征，并在未来进行提取物、馏分或分离化合物的定量分析和生物活性研究。这是关于传统使用的稀有分类群和其生物合成潜力的体外培养的首次报告。
