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基于脂质体的心肌肌钙蛋白 I 检测免疫分析的建立。

Development of Liposome-Based Immunoassay for the Detection of Cardiac Troponin I.

机构信息

Department of Biology, Chemistry, and Environmental Sciences, American University of Sharjah, Sharjah 26666, United Arab Emirates.

出版信息

Molecules. 2021 Nov 19;26(22):6988. doi: 10.3390/molecules26226988.

DOI:10.3390/molecules26226988
PMID:34834080
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8623906/
Abstract

Cardiovascular diseases (CVDs) are one of the foremost causes of mortality in intensive care units worldwide. The development of a rapid method to quantify cardiac troponin I (cTnI)-the gold-standard biomarker of myocardial infarction (MI) (or "heart attack")-becomes crucial in the early diagnosis and treatment of myocardial infarction (MI). This study investigates the development of an efficient fluorescent "sandwich" immunoassay using liposome-based fluorescent signal amplification and thereby enables the sensing and quantification of serum-cTnI at a concentration relevant to clinical settings. The calcein-loaded liposomes were utilized as fluorescent nano vehicles, and these have exhibited appropriate stability and efficient fluorescent properties. The standardized assay was sensitive and selective towards cTnI in both physiological buffer solutions and spiked human serum samples. The novel assay presented noble analytical results with sound dynamic linearity over a wide concentration range of 0 to 320 ng/mL and a detection limit of 6.5 ng/mL for cTnI in the spiked human serum.

摘要

心血管疾病（CVDs）是全球重症监护病房中首要的死亡原因之一。开发一种快速定量检测心肌肌钙蛋白 I（cTnI）的方法变得至关重要，cTnI 是心肌梗死（MI）（或“心脏病发作”）的金标准生物标志物。本研究开发了一种基于脂质体荧光信号放大的高效荧光“三明治”免疫分析法，从而能够在与临床相关的浓度下检测和定量血清 cTnI。负载钙黄绿素的脂质体可用作荧光纳米载体，具有适当的稳定性和高效的荧光性能。该标准化测定法对生理缓冲液和加标人血清样本中的 cTnI 具有灵敏和选择性。新型测定法在广泛的浓度范围内（0 至 320ng/mL）呈现出优异的分析结果，并且在加标人血清中的检测限为 6.5ng/mL。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7219/8623906/8fd79f5d1d3b/molecules-26-06988-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7219/8623906/0555f7ac778a/molecules-26-06988-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7219/8623906/01ba5a18e049/molecules-26-06988-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7219/8623906/5bd128216513/molecules-26-06988-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7219/8623906/2f02d87227e3/molecules-26-06988-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7219/8623906/bc33d63be5f4/molecules-26-06988-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7219/8623906/201154ce0289/molecules-26-06988-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7219/8623906/8fd79f5d1d3b/molecules-26-06988-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7219/8623906/0555f7ac778a/molecules-26-06988-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7219/8623906/01ba5a18e049/molecules-26-06988-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7219/8623906/5bd128216513/molecules-26-06988-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7219/8623906/2f02d87227e3/molecules-26-06988-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7219/8623906/bc33d63be5f4/molecules-26-06988-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7219/8623906/201154ce0289/molecules-26-06988-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7219/8623906/8fd79f5d1d3b/molecules-26-06988-g006.jpg

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Biosensors (Basel). 2021 Feb 14;11(2):49. doi: 10.3390/bios11020049.
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