School of Chemistry and Environmental Engineering, Hubei Minzu University, Enshi, 445000, Hubei, PR China.
School of Chemistry and Environmental Engineering, Hubei Minzu University, Enshi, 445000, Hubei, PR China.
Talanta. 2024 Nov 1;279:126576. doi: 10.1016/j.talanta.2024.126576. Epub 2024 Jul 15.
Herein, we developed a platinum-copper nano-enzyme-linked immunosorbent assay (NLISA) based split diagnostic platform for the ultrasensitive detection of cardiac troponin I (cTnI). The PtCu nanozyme synthesized by one-pot synthesis exhibited ultra-high peroxidase-like activity (35.17 U mg), which was about 4.5 times higher than that of the unmodified Pt nanozyme (8.83 U mg). Due to the efficient peroxidase-like activity of the copper-platinum complexed nanozyme, transduction and sequential amplification of cTnI biological signals were achieved in combination with a liposome-embedded amplification strategy. The encapsulation efficiency was calculated by introducing a liposomal bilayer model, which showed that the introduction of a single liposomal molecule could amplify the signal up to 870-fold, thus promising a high sensitivity test. Notably, the dynamic response of cTnI was in the range of 0.1-5000 pg mL with an ultra-low detection limit (0.048 pg mL). The developed NLISA analysis system provides a new way to discover efficient and sensitive alternatives to ELISA kits, which can meet the practical needs of community healthcare testing conditions and rapid testing in hospitals.
在此，我们开发了一种基于铂铜纳米酶联免疫吸附测定（NLISA）的分裂诊断平台，用于超灵敏检测心肌肌钙蛋白 I（cTnI）。一锅合成法合成的 PtCu 纳米酶表现出超高过氧化物酶样活性（35.17 U·mg），约为未修饰 Pt 纳米酶（8.83 U·mg）的 4.5 倍。由于铜-铂配合物纳米酶的高效过氧化物酶样活性，与脂质体嵌入扩增策略相结合，实现了 cTnI 生物信号的转导和顺序放大。通过引入脂质体双层模型计算包封效率，表明引入单个脂质体分子可将信号放大至 870 倍，从而有望实现高灵敏度测试。值得注意的是，cTnI 的动力学响应范围为 0.1-5000 pg·mL，检测下限超低（0.048 pg·mL）。所开发的 NLISA 分析系统为发现高效灵敏的 ELISA 试剂盒替代品提供了一种新方法，可满足社区医疗保健检测条件和医院快速检测的实际需求。
