Faculty of Sciences, Cairo University, Giza 12613, Egypt.
Biology Department, Faculty of Science, King Khalid University, Abha 9004, Saudi Arabia.
Molecules. 2021 Nov 22;26(22):7057. doi: 10.3390/molecules26227057.
We aimed to evaluate the anticancer potential of crude venom (CV), γ irradiated venom (IRRV), and propolis ethanolic extract (PEE). IRRV showed a higher toxicity than CV, while CV-PEE showed higher toxicity than IRRV and CV against lung [A549] and prostate [PC3] cancer cells. Toxicity to [A549] and [PC3] cells was concentration and cell type dependent. In comparison to controls, apoptotic genes showed a significant upregulation of P53 and Casp-3 and a downregulation of Bcl-2. Also, induced elevated DNA accumulation in the [S] phase post PC3 cell treatment with IRRV and CV, as well as a significant DNA accumulation at G2/M phase after IRRV treatment of A549 cells. In contrast, PC3 cells showed a negligible cellular DNA accumulation after PEE treatment. Glutathione reductase [GR] was reduced in case of PC3 and A549 cell treated with IRRV, CV, and PEE compared with its values in untreated cell control. The Malondialdehyde [MDA] values in both cells recorded a significant elevation post IRRV treatment compared to the rest of the treatment regimen and untreated cell control. Similarly, IRRV and CV-PEE mix showed obviously higher reactive oxygen species [ROS] values than PC3 and A549 cell treatments with CV and PEE.
我们旨在评估粗毒液（CV）、γ 辐照毒液（IRRV）和蜂胶乙醇提取物（PEE）的抗癌潜力。IRRV 的毒性高于 CV，而 CV-PEE 对肺癌 [A549] 和前列腺癌 [PC3] 细胞的毒性高于 IRRV 和 CV。细胞毒性与浓度和细胞类型有关。与对照组相比，凋亡基因显示 P53 和 Caspase-3 的显著上调以及 Bcl-2 的下调。此外，IRRV 和 CV 处理 PC3 细胞后，S 期的 DNA 积累显著增加，IRRV 处理 A549 细胞后 G2/M 期的 DNA 积累也显著增加。相比之下，PEE 处理后 PC3 细胞的细胞 DNA 积累可忽略不计。与未经处理的细胞对照相比，IRRV、CV 和 PEE 处理的 PC3 和 A549 细胞中的谷胱甘肽还原酶 [GR] 减少。与其他处理方案和未经处理的细胞对照相比，IRRV 处理后两种细胞中的丙二醛 [MDA] 值均显著升高。同样，IRRV 和 CV-PEE 混合物显示出比 CV 和 PEE 处理 PC3 和 A549 细胞更高的活性氧 [ROS] 值。
