Gonzalez Juan H, Taylor Joseph S, Reed Kelsey M, Wright R Clay, Bargmann Bastiaan O R
School of Plant and Environmental Sciences, Virginia Polytechnic Institute and State University (Virginia Tech), Blacksburg, VA 24061, USA.
Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University (Virginia Tech), Blacksburg, VA 24061, USA.
Plants (Basel). 2021 Oct 23;10(11):2271. doi: 10.3390/plants10112271.
Regeneration of fertile plants from tissue culture is a critical bottleneck in the application of new plant breeding technologies. Ectopic overexpression of morphogenic factors is a promising workaround for this hurdle.
Conditional overexpression of and Δ was used to study the effect of timing the overexpression of these morphogenic factors during shoot regeneration from root explants in Arabidopsis. In addition, their effect on auxin-signaling activation was examined by visualization and cytometric quantification of the DR5:GFP auxin-signaling reporter in roots and protoplasts, respectively.
The induced expression of both and Δ led to an activation of auxin signaling in roots. Activation of auxin signaling by and Δ was further quantified by transient transformation of protoplasts. Ectopic overexpression of both and Δ enhanced regeneration efficiency, but only during the shoot-induction stage of regeneration and not during the callus-induction stage.
The overexpression of and Δ both lead to activation of auxin signaling. Expression during the shoot-induction stage is critical for the enhancement of shoot regeneration by and Δ.
从组织培养中再生可育植株是新植物育种技术应用中的一个关键瓶颈。形态发生因子的异位过表达是克服这一障碍的一种有前景的方法。
利用 和Δ的条件性过表达来研究在拟南芥根外植体芽再生过程中这些形态发生因子过表达时间的影响。此外，分别通过可视化和细胞计数定量分析根和原生质体中DR5:GFP生长素信号报告基因，研究它们对生长素信号激活的影响。
和Δ的诱导表达均导致根中生长素信号的激活。通过原生质体的瞬时转化进一步定量分析了 和Δ对生长素信号的激活作用。 和Δ的异位过表达均提高了再生效率，但仅在再生的芽诱导阶段，而不是在愈伤组织诱导阶段。
和Δ的过表达均导致生长素信号的激活。在芽诱导阶段的表达对于 和Δ增强芽再生至关重要。
