Kishor D S, Alavilli Hemasundar, Lee Sang-Choon, Kim Jeong-Gu, Song Kihwan
Department of Bioresources Engineering, Sejong University, Seoul 05006, Korea.
PHYZEN, Seongnam-si 13558, Gyeonggi-do, Korea.
Plants (Basel). 2021 Oct 29;10(11):2341. doi: 10.3390/plants10112341.
Despite various efforts in identifying the genes governing the white immature fruit skin color in cucumber, the genetic basis of the white immature fruit skin color is not well known. In the present study, genetic analysis showed that a recessive gene confers the white immature fruit skin-color phenotype over the light-green color of a Korean slicer cucumber. High-throughput QTL-seq combined with bulked segregation analysis of two pools with the extreme phenotypes (white and light-green fruit skin color) in an F population identified two significant genomic regions harboring QTLs for white fruit skin color within the genomic region between 34.1 and 41.67 Mb on chromosome 3, and the genomic region between 12.2 and 12.7 Mb on chromosome 5. Further, nonsynonymous SNPs were identified with a significance of < 0.05 within the QTL regions, resulting in eight homozygous variants within the QTL region on chromosome 3. SNP marker analysis uncovered the novel missense mutations in and genes and showed consistent results with the phenotype of light-green and white fruit skin-colored F plants. These two genes were located 0.5 Mb apart on chromosome 3, which are considered strong candidate genes. Altogether, this study laid a solid foundation for understanding the genetic basis and marker-assisted breeding of immature fruit skin color in cucumber.
尽管在鉴定控制黄瓜白色未成熟果实果皮颜色的基因方面做出了各种努力，但白色未成熟果实果皮颜色的遗传基础仍不清楚。在本研究中，遗传分析表明，一个隐性基因使韩国切片黄瓜的白色未成熟果实果皮颜色表型相对于浅绿色表型得以显现。高通量QTL测序结合F群体中两个具有极端表型（白色和浅绿色果皮颜色）的池的混合分离分析，在3号染色体上34.1至41.67 Mb的基因组区域以及5号染色体上12.2至12.7 Mb的基因组区域内，鉴定出两个含有白色果皮颜色QTL的重要基因组区域。此外，在QTL区域内鉴定出非同义SNP，其显著性<0.05，在3号染色体的QTL区域内产生了8个纯合变体。SNP标记分析揭示了 和 基因中的新错义突变，并与浅绿色和白色果皮颜色的F植株的表型显示出一致的结果。这两个基因在3号染色体上相距0.5 Mb，被认为是强有力的候选基因。总之，本研究为理解黄瓜未成熟果实果皮颜色的遗传基础和标记辅助育种奠定了坚实的基础。
