Park Jin-Cheon, Yoon Young-Mi, Lee Chang-Hyun, Hur On-Sook, Kim Sang-Min
Winter Crop Research Division, Department of Crop Science, National Institute of Crop and Food Science, Rural Development Administration, Wanju, Republic of Korea.
Crop Environment Research Division, Department of Crop Science, National Institute of Crop and Food Science, Rural Development Administration, Wanju, Republic of Korea.
Front Plant Sci. 2025 May 12;16:1596811. doi: 10.3389/fpls.2025.1596811. eCollection 2025.
Powdery mildew (PM) caused by f.sp. is a major fungal disease affecting barley ( L.). The most effective approach to controlling this disease is the development of resistant cultivars. In this study, we investigated the genomic regions associated with PM resistance by performing quantitative loci sequencing (QTL-seq) twice using the parental lines 'Hopum' (susceptible) and 'Jeonju 182' (resistant) as reference genomes.
This study was conducted from 2022 to 2024 at the National Institute of Agricultural Sciences in Wanju, Republic of Korea. We conducted artificial crossing, genomic DNA extraction, phenotypic evaluation, QTL-seq analysis, and cleaved amplified polymorphic sequence (CAPS) marker development. Candidate gene expression was analyzed using real-time quantitative reverse transcription PCR.
A total of 2,130 common variants were identified in two regions of chromosome 1H (6,940,595-18,008,713 bp and 19,363,700-20,551,018 bp). Twenty-one non-synonymous single nucleotide polymorphisms among these variants were used to develop CAPS markers, which were validated in an F population and malting barley cultivars. The PMC_75 marker, which is annotated as , showed a strong association with resistance and was highly expressed in 'Jeonju 182.' This marker is associated with a Clathrin Assembly Protein, which is involved in vesicle formation and intracellular trafficking, processes essential for cellular signaling and defense responses.
The development of the CAPS marker (PMC_75) provides a valuable tool for marker-assisted selection in breeding PM-resistant malting barley, improving breeding efficiency, and accelerating the development of resistant cultivars.
由白粉菌引起的白粉病是影响大麦（Hordeum vulgare L.）的一种主要真菌病害。控制这种病害最有效的方法是培育抗病品种。在本研究中，我们以亲本系“霍普姆”（感病）和“全州182”（抗病）作为参考基因组，通过两次进行数量性状位点测序（QTL-seq）来研究与白粉病抗性相关的基因组区域。
本研究于2022年至2024年在韩国完州的国家农业科学研究所进行。我们进行了人工杂交、基因组DNA提取、表型评估、QTL-seq分析以及酶切扩增多态性序列（CAPS）标记开发。使用实时定量逆转录PCR分析候选基因表达。
在1H染色体的两个区域（6,940,595 - 18,008,713 bp和19,363,700 - 20,551,018 bp）共鉴定出2,130个常见变异。这些变异中的21个非同义单核苷酸多态性被用于开发CAPS标记，这些标记在一个F群体和麦芽大麦品种中得到验证。注释为的PMC_75标记与抗性表现出强烈关联，并且在“全州182”中高表达。该标记与一种网格蛋白组装蛋白相关，该蛋白参与囊泡形成和细胞内运输，这些过程对于细胞信号传导和防御反应至关重要。
CAPS标记（PMC_75）的开发为培育抗白粉病麦芽大麦的标记辅助选择、提高育种效率以及加速抗病品种的开发提供了有价值的工具。
