Ma Li-Ting, Liu Pi-Ling, Cheng Yang-Tui, Shiu Tz-Fan, Chu Fang-Hua
School of Forestry and Resource Conservation, National Taiwan University, Taipei 10617, Taiwan.
Plants (Basel). 2021 Nov 8;10(11):2404. doi: 10.3390/plants10112404.
is a monotypic species, and its terpenoid-rich property has been reported in recent years. To uncover monoterpene biosynthesis in , this study used transcriptome mining to identify candidates with tentative monoterpene synthase activity. Along with the phylogenetic analysis and in vitro assay, two geraniol synthases (TPS13 and TPS14), a linalool synthase (TPS15), and a β-pinene synthase (TPS16), were functionally characterized. Via the comparison of catalytic residues, the Cys/Ser at region 1 might be crucial in determining the formation of α-pinene or β-pinene. In addition, the Cupressaceae monoterpene synthases were phylogenetically clustered together; they are unique and different from those of published conifer species. In summary, this study aimed to uncover the ambiguous monoterpenoid network in , which would expand the landscape of monoterpene biosynthesis in Cupressaceae species.
是一个单型物种，其富含萜类化合物的特性近年来已有报道。为了揭示该物种中单萜生物合成的过程，本研究利用转录组挖掘来鉴定具有初步单萜合酶活性的候选基因。通过系统发育分析和体外测定，对两种香叶醇合酶（TPS13和TPS14）、一种芳樟醇合酶（TPS15）和一种β-蒎烯合酶（TPS16）进行了功能表征。通过比较催化残基，区域1中的半胱氨酸/丝氨酸可能对决定α-蒎烯或β-蒎烯的形成至关重要。此外，柏科单萜合酶在系统发育上聚集在一起；它们是独特的，与已发表的针叶树种不同。总之，本研究旨在揭示该物种中模糊的单萜类化合物网络，这将扩展柏科物种中单萜生物合成的研究范围。
