Ho Chen-Lung, Yang Su-Sing, Chang Tsong-Min, Su Yu-Chang
Division of Wood Cellulose, Taiwan Forestry Research Institute, 53, Nanhai Rd, Taipei, Taiwan 100.
Nat Prod Commun. 2012 Feb;7(2):261-4.
This study investigated the chemical composition, antioxidant, antimicrobial and anti-wood-decay fungal activities of the essential oil isolated from the twigs of Taiwania cryptomerioides from Taiwan. The essential oil was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. A total of 35 compounds were identified, representing 100% of the oil. The main components identified were alpha-cadinol (45.9%), ferruginol (18.9%) and beta-eudesmol (10.8%). The antioxidant activity of the oil was tested by the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging capability test. The results showed an IC50 of 90.8 +/- 0.2 microg/mL. The active source compound was ferruginol. The antimicrobial activity of the oil was tested by the disc diffusion and micro-broth dilution methods against ten microbial species. The oil exhibited strong growth suppression against Gram-positive bacteria and yeast with inhibition zones of 45-52 mm and MIC values of 31.25-62.5 microg/mL, respectively. The anti-wood-decay fungal activity of the oil was also evaluated. The oil demonstrated excellent activity against four wood-decay-fungal species. For the antimicrobial and anti-wood-decay fungal activities of the oil, the active source compounds were determined to be alpha-cadinol, beta-eudesmol and ferruginol.
本研究调查了从台湾台湾杉小枝中分离得到的精油的化学成分、抗氧化、抗菌及抗木材腐朽真菌活性。采用Clevenger型装置通过水蒸馏法分离精油，并通过气相色谱-氢火焰离子化检测器（GC-FID）和气相色谱-质谱联用仪（GC-MS）对其进行表征。共鉴定出35种化合物，占精油总量的100%。鉴定出的主要成分是α-杜松醇（45.9%）、铁锈醇（18.9%）和β-桉叶醇（10.8%）。通过2,2-二苯基-1-苦基肼（DPPH）自由基清除能力试验测试了该精油的抗氧化活性。结果显示其半数抑制浓度（IC50）为90.8±0.2微克/毫升。活性源化合物为铁锈醇。采用纸片扩散法和微量肉汤稀释法测试了该精油对10种微生物的抗菌活性。该精油对革兰氏阳性菌和酵母菌表现出较强的生长抑制作用，抑菌圈直径分别为45 - 52毫米，最低抑菌浓度（MIC）值分别为31.25 - 62.5微克/毫升。还评估了该精油的抗木材腐朽真菌活性。该精油对4种木材腐朽真菌表现出优异的活性。对于该精油的抗菌和抗木材腐朽真菌活性，确定活性源化合物为α-杜松醇、β-桉叶醇和铁锈醇。
