Wang Yiru, Zhang Junli, Sun Minghao, He Cheng, Yu Ke, Zhao Bing, Li Rui, Li Jian, Yang Zongying, Wang Xiao, Duan Haiyang, Fu Junjie, Liu Sanzhen, Zhang Xuebin, Zheng Jun
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
State Key Laboratory of Crop Stress Adaptation and Improvement, Henan Joint International Laboratory for Crop Multi-Omics Research, School of Life Sciences, Henan University, Kaifeng 475000, China.
Plants (Basel). 2021 Nov 12;10(11):2437. doi: 10.3390/plants10112437.
Maize vivipary, precocious seed germination on the ear, affects yield and seed quality. The application of multi-omics approaches, such as transcriptomics or metabolomics, to classic vivipary mutants can potentially reveal the underlying mechanism. Seven maize vivipary mutants were selected for transcriptomic and metabolomic analyses. A suite of transporters and transcription factors were found to be upregulated in all mutants, indicating that their functions are required during seed germination. Moreover, vivipary mutants exhibited a uniform expression pattern of genes related to abscisic acid (ABA) biosynthesis, gibberellin (GA) biosynthesis, and ABA core signaling. 4 (), which is involved in ABA biosynthesis, was markedly downregulated and () was upregulated in all vivipary mutants, indicating antagonism between these two phytohormones. The ABA core signaling components (PYL-ABI1-SnRK2-ABI3) were affected in most of the mutants, but the expression of these genes was not significantly different between the mutant and wild-type seeds. Metabolomics analysis integrated with co-expression network analysis identified unique metabolites, their corresponding pathways, and the gene networks affected by each individual mutation. Collectively, our multi-omics analyses characterized the transcriptional and metabolic landscape during vivipary, providing a valuable resource for improving seed quality.
玉米穗发芽，即种子在穗上过早萌发，会影响产量和种子质量。将转录组学或代谢组学等多组学方法应用于经典的穗发芽突变体，有可能揭示其潜在机制。选择了7个玉米穗发芽突变体进行转录组学和代谢组学分析。发现一组转运蛋白和转录因子在所有突变体中均上调，表明它们的功能在种子萌发过程中是必需的。此外，穗发芽突变体在与脱落酸（ABA）生物合成、赤霉素（GA）生物合成和ABA核心信号传导相关的基因上表现出一致的表达模式。参与ABA生物合成的4（）在所有穗发芽突变体中显著下调，而（）上调，表明这两种植物激素之间存在拮抗作用。大多数突变体中的ABA核心信号成分（PYL-ABI1-SnRK2-ABI3）受到影响，但这些基因在突变体和野生型种子之间的表达没有显著差异。代谢组学分析与共表达网络分析相结合，鉴定出了独特的代谢物、它们相应的途径以及受每个单独突变影响的基因网络。总的来说，我们的多组学分析描绘了穗发芽过程中的转录和代谢图景，为提高种子质量提供了宝贵资源。
