Yang Dongjie, Liu Yuanyuan, Cheng Hailiang, Wang Qiaolian, Lv Limin, Zhang Youping, Zuo Dongyun, Song Guoli
Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, China.
Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, China.
Plants (Basel). 2021 Nov 15;10(11):2461. doi: 10.3390/plants10112461.
Amino acid transporters () are a kind of membrane proteins that mediate the transport of amino acids across cell membranes in higher plants. The AAT proteins are involved in regulating plant cell growth and various developmental processes. However, the biological function of this gene family in cotton fiber development is not clear. In this study, 190, 190, 101, and 94 full-length genes were identified from , , , and . A total of 575 genes from the four cotton species were divided into two subfamilies and 12 clades based on phylogenetic analysis. The genes in the four cotton species were distributed on all the chromosomes. All genes contain multiple exons, and each GhAAT protein has multiple conserved motifs. Transcriptional profiling and RT qPCR analysis showed that four genes tend to express specifically at the fiber initiation stage. Eight genes tend to express specifically at the fiber elongation and maturity stage, and four genes tend to express specifically at the fiber initiation and elongation stages. Our results provide a solid basis for further elucidating the biological function of genes related to cotton fiber development and offer valuable genetic resources for crop improvement in the future.
氨基酸转运蛋白（AATs）是一类膜蛋白，介导高等植物中氨基酸跨细胞膜的运输。AAT蛋白参与调节植物细胞生长和各种发育过程。然而，该基因家族在棉花纤维发育中的生物学功能尚不清楚。在本研究中，从陆地棉、海岛棉、亚洲棉和草棉中分别鉴定出190个、190个、101个和94个AAT全长基因。基于系统发育分析，将来自这四个棉花物种的总共575个AAT基因分为两个亚家族和12个进化枝。四个棉花物种中的AAT基因分布在所有染色体上。所有AAT基因都包含多个外显子，并且每个GhAAT蛋白都有多个保守基序。转录谱分析和RT-qPCR分析表明，四个AAT基因倾向于在纤维起始阶段特异性表达。八个基因倾向于在纤维伸长和成熟阶段特异性表达，四个基因倾向于在纤维起始和伸长阶段特异性表达。我们的结果为进一步阐明与棉花纤维发育相关的AAT基因的生物学功能提供了坚实的基础，并为未来作物改良提供了有价值的遗传资源。
