Division of Infectious Diseases, Department of Medicine, University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA.
Joint Clinical Research Centre, Kampala P.O. Box 10005, Uganda.
Viruses. 2021 Oct 22;13(11):2135. doi: 10.3390/v13112135.
Women with HIV may experience higher rates of non-AIDS comorbidities compared to men with HIV, but the underlying mechanisms are not well understood. We investigated sex-related differences in the effects of HIV on monocyte phenotypes within the Ugandan Study of HIV effects on the Myocardium and Atherosclerosis (mUTIMA). Of 133 participants who provided blood for flow cytometry assays, 86 (65%) were women and 91 (68%) were persons living with HIV (PLWH) on antiretroviral therapy. The median age was 57 (interquartile range, 52-63) years. PLWH exhibited a lower proportion of circulating CD14CD16 classical monocytes (66.3% vs. 75.1%; < 0.001), and higher proportion of CD14CD16 inflammatory monocytes (17% vs. 11.7%; = 0.005) compared to HIV-uninfected participants. PLWH had an increased expression of the chemokine receptor CX3CR1 in total monocytes (CX3CR1 monocytes, 24.5% vs. 4.7%; < 0.001) and monocyte subsets. These findings were generally similar when analyzed by sex, with no significant interactions between sex and HIV status in adjusted models. Our data show that the inflammatory monocyte subset is expanded and monocyte CX3CR1 chemokine receptor expression is enhanced among PLWH, regardless of sex. Whether these parameters differentially affect risk for non-AIDS comorbidities and clinical outcomes in women with HIV requires additional investigation.
与男性 HIV 感染者相比，HIV 女性感染者可能经历更高的非艾滋病合并症发生率，但潜在机制尚不清楚。我们在乌干达 HIV 对心肌和动脉粥样硬化影响研究（mUTIMA）中调查了 HIV 对单核细胞表型的影响在性别方面的差异。在提供流式细胞术检测血液的 133 名参与者中，86 名（65%）为女性，91 名（68%）为接受抗逆转录病毒治疗的 HIV 感染者（PLWH）。中位年龄为 57 岁（四分位距，52-63）。PLWH 循环中 CD14CD16 经典单核细胞的比例较低（66.3% vs. 75.1%；<0.001），CD14CD16 炎症性单核细胞的比例较高（17% vs. 11.7%；=0.005），与 HIV 未感染者相比。PLWH 中总单核细胞（CX3CR1 单核细胞，24.5% vs. 4.7%；<0.001）和单核细胞亚群中趋化因子受体 CX3CR1 的表达增加。在按性别进行分析时，这些发现通常相似，在调整后的模型中，性别和 HIV 状态之间没有显著的相互作用。我们的数据表明，炎症性单核细胞亚群在 PLWH 中扩张，单核细胞 CX3CR1 趋化因子受体表达增强，无论性别如何。这些参数是否会对 HIV 女性患者的非艾滋病合并症和临床结局风险产生不同影响，需要进一步研究。
