Research Centre for Infectious Diseases, Department of Molecular and Biomedical Sciences, School of Biological Sciences, The University of Adelaide, Adelaide 5005, Australia.
Adelaide Medical School, The University of Adelaide, Adelaide 5000, Australia.
Viruses. 2021 Oct 26;13(11):2157. doi: 10.3390/v13112157.
Understanding the dynamic relationship between viral pathogens and cellular host factors is critical to furthering our knowledge of viral replication, disease mechanisms and development of anti-viral therapeutics. CRISPR genome editing technology has enhanced this understanding, by allowing identification of pro-viral and anti-viral cellular host factors for a wide range of viruses, most recently the cause of the COVID-19 pandemic, SARS-CoV-2. This review will discuss how CRISPR knockout and CRISPR activation genome-wide screening methods are a robust tool to investigate the viral life cycle and how other class 2 CRISPR systems are being repurposed for diagnostics.
了解病毒病原体和细胞宿主因素之间的动态关系对于深入了解病毒复制、疾病机制和开发抗病毒治疗方法至关重要。CRISPR 基因组编辑技术通过允许鉴定广泛的病毒（最近是导致 COVID-19 大流行的 SARS-CoV-2 病毒）的前病毒和抗病毒细胞宿主因子，增强了我们对此的理解。这篇综述将讨论 CRISPR 敲除和 CRISPR 激活全基因组筛选方法如何成为研究病毒生命周期的强大工具，以及其他 2 类 CRISPR 系统如何被重新用于诊断。
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