Microbiologie Environnementale Biotechnologie, Institut Méditerranéen d'Océanologie, 163 Avenue de Luminy, 13009 Marseille, France.
Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, France.
Viruses. 2021 Oct 26;13(11):2163. doi: 10.3390/v13112163.
Despite a surge of RNA virome sequencing in recent years, there are still many RNA viruses to uncover-as indicated by the relevance of viral dark matter to RNA virome studies (i.e., putative viruses that do not match to taxonomically identified viruses). This study explores a unique site, a high-rate algal pond (HRAP), for culturing industrially microalgae, to elucidate new RNA viruses. The importance of viral-host interactions in aquatic systems are well documented, and the ever-expanding microalgae industry is no exception. As the industry becomes a more important source of sustainable plastic manufacturing, a producer of cosmetic pigments and alternative protein sources, and a means of CO remediation in the face of climate change, studying microalgal viruses becomes a vital practice for proactive management of microalgae cultures at the industrial level. This study provides evidence of RNA microalgal viruses persisting in a CO remediation pilot project HRAP and uncovers the diversity of the RNA virosphere contained within it. Evidence shows that family is cultured in the basin, alongside other potential microalgal infecting viruses (e.g., family , family , and family ). Finally, we demonstrate that the RNA viral diversity of the HRAP is temporally dynamic across two successive culturing seasons.
尽管近年来 RNA 病毒组测序技术有了突飞猛进的发展，但仍有许多 RNA 病毒有待发现——这表明病毒暗物质与 RNA 病毒组研究密切相关（即，那些与分类学上已识别的病毒不匹配的假定病毒）。本研究探索了一个独特的地点，即一个高藻池塘（HRAP），用于培养工业微藻，以阐明新的 RNA 病毒。病毒-宿主相互作用在水生系统中的重要性已有充分记录，而不断扩大的微藻产业也不例外。随着该产业成为可持续塑料制造、化妆品颜料和替代蛋白质来源的更重要来源，以及应对气候变化时 CO 修复的手段，研究微藻病毒成为在工业水平上对微藻培养进行主动管理的重要实践。本研究提供了证据表明，RNA 微藻病毒在 CO 修复试点项目 HRAP 中持续存在，并揭示了其中包含的 RNA 病毒圈的多样性。有证据表明，科在盆中与其他潜在的感染微藻的病毒（例如科、科和科）一起被培养。最后，我们证明 HRAP 的 RNA 病毒多样性在两个连续的养殖季节中具有时间动态性。
