Lim Jongwon, Hong Suhee
Department of Marine Biotechnology, Gangneung-Wonju National University, Gangneung 25457, Korea.
Vaccines (Basel). 2021 Oct 22;9(11):1234. doi: 10.3390/vaccines9111234.
This study aimed to identify the molecular mechanisms regulated by a combined vaccine against and (O1 serotype). These bacteria cause furunculosis and vibriosis, respectively, and are associated with a high mortality in rainbow trout in Korea. The vaccine upregulated gene expression of TCRα, T-bet, sIgM, and mIgM, markers of an activated adaptive immune response. On days 1, 3, and 5, transcriptome analysis revealed 862 (430 up- and 432 downregulated), 492 (204 up- and 288 downregulated), and 741 (270 up- and 471 downregulated) differentially expressed genes (DEGs), respectively. Gene ontology (GO) enrichment analysis identified 377 (108 MF, 132 CC, 137 BP), 302 (60 MF, 180 CC, 62 BP), and 314 (115 MF, 129 CC, 70 BP) GOs at days 1, 3, and 5, respectively. Kyoto Encyclopedia of Genetic and Genomic enrichment analysis identified eight immune system-related pathways like cytokine-cytokine receptor interaction, NF-kappaB signaling pathway, TNF signaling pathway, NOD-like receptor signaling pathway, cytosolic DNA sensing pathway, cell adhesion molecule, complement and coagulation cascade, and antigen processing and presentation. In the analysis of the protein-protein interaction of immune-related DEGs, a total of 59, 21, and 21 interactional relationships were identified at days 1, 3, and 5, respectively, with TNF having the highest centrality at all three time points.
本研究旨在确定一种针对[细菌名称1]和[细菌名称2]（O1血清型）的联合疫苗所调控的分子机制。这些细菌分别导致疖病和弧菌病，并且与韩国虹鳟鱼的高死亡率相关。该疫苗上调了TCRα、T-bet、sIgM和mIgM的基因表达，这些都是活化的适应性免疫反应的标志物。在第1天、第3天和第5天，转录组分析分别揭示了862个（430个上调和432个下调）、492个（204个上调和288个下调）和741个（270个上调和471个下调）差异表达基因（DEG）。基因本体（GO）富集分析在第1天、第3天和第5天分别鉴定出377个（108个分子功能，132个细胞组分，137个生物学过程）、302个（60个分子功能，180个细胞组分，62个生物学过程）和314个（115个分子功能，129个细胞组分，70个生物学过程）GO。京都基因与基因组百科全书富集分析确定了八个与免疫系统相关的途径，如细胞因子-细胞因子受体相互作用、NF-κB信号通路、TNF信号通路、NOD样受体信号通路、胞质DNA传感通路、细胞粘附分子、补体和凝血级联以及抗原加工和呈递。在对免疫相关DEG的蛋白质-蛋白质相互作用分析中，在第1天、第3天和第5天分别鉴定出总共59个、21个和21个相互作用关系，其中TNF在所有三个时间点的中心性最高。
