Pathology Department, College of Veterinary Medicine, Suez Canal Univ., Egypt.
Department of Aquatic Animal Medicine & Management , College of Veterinary Medicine, Cairo Univ., Egypt.
Fish Shellfish Immunol. 2021 Apr;111:145-151. doi: 10.1016/j.fsi.2020.10.011. Epub 2020 Nov 12.
This experimental studies investigated the protective efficiencies and the potential immune mechanisms of vibrio monovalent and polyvalent autogenous formalin-inactivated whole-cell bacterins (FKC) in Gilthead sea bream (Sparus aurata) cultured in Egypt. Two months post-vaccination, the relative percentage survival (RPS) was estimated after challenge with the vaccine's homologues pathogenic strains. The survival values were 100% and 83.3% in groups immunized with monovalent V. alginolyticus or V. parahaemolyticus FKC bacterins, respectively. On the other hand, survival values were 91.75% and 75% in fish groups subjected to polyvalent (V. parahaemolyticus O11: K40 & V. alginolyticus) and (V. parahaemolyticus O3: K6 & V. alginolyticus) FKC bacterins, respectively. Overall, the tested vaccine preparations were significantly increased (P < 0.05) the agglutination antibody titer, phagocytic activity, respiratory burst activity, when compared to the non-immunized control group. The current results conclude that, autogenous Vibrio vaccines provoked a promising protection against vibriosis in Gilthead sea bream cultured in Egypt, it was superior in monovalent FKC V. alginolyticus vaccine and polyvalent FKC of V. parahaemolyticus O11: K40 with V. alginolyticus vaccine that could be useful means of prevention and control of vibriosis.
本实验研究了单价和多价副溶血性弧菌单价和多价福尔马林灭活全细胞菌苗（FKC）对埃及养殖真鲷（Sparus aurata）的保护效率和潜在免疫机制。免疫后 2 个月，用疫苗同源致病性菌株进行攻毒后，估计相对存活率（RPS）。用单价 V. alginolyticus 或 V. parahaemolyticus FKC 菌苗免疫的鱼群的存活率分别为 100%和 83.3%。另一方面，在接受多价（V. parahaemolyticus O11：K40 和 V. alginolyticus）和（V. parahaemolyticus O3：K6 和 V. alginolyticus）FKC 菌苗的鱼群中，存活率分别为 91.75%和 75%。总的来说，与未免疫对照组相比，试验疫苗制剂显著提高了（P<0.05）凝集抗体滴度、吞噬活性和呼吸爆发活性。目前的结果表明，副溶血性弧菌自主疫苗在埃及养殖的真鲷中引起了对抗弧菌病的有希望的保护作用，在单价 FKC V. alginolyticus 疫苗和多价 FKC 的 V. parahaemolyticus O11：K40 与 V. alginolyticus 疫苗中效果更好，这可能是预防和控制弧菌病的有用手段。
