Meng Xianghu, Chi Heng, Zhang Zuobing, Li Qian, Sheng Xiuzhen, Tang Xiaoqian, Xing Jing, Zhan Wenbin
Laboratory of Pathology and Immunology of Aquatic Animals, KLMME, Ocean University of China, Qingdao 266003, China.
Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.
Vaccines (Basel). 2023 Oct 16;11(10):1603. doi: 10.3390/vaccines11101603.
() is a bacterium that seriously harms flounder and other aquaculture species. Vaccination is an effective means of preventing vibriosis and is mainly administered by intraperitoneal injection. Effective antigen processing at the initial stage of immunization is essential to elicit adaptive immune responses and improve vaccine efficacy. To understand the early immune response of flounder caused by inactivated , we detected the transcriptome profiles of the cells in the peritoneal cavity (PerCs) after inactivated immunization. More than 10 billion high-quality reads were obtained, of which about 89.33% were successfully mapped to the reference genome of flounder. A total of 1985, 3072, 4001, and 5476 differentially expressed genes were captured at 6, 12, 24, and 48 h post immunization, respectively. The hub module correlated with the immunization time was identified by WGCNA. GO and KEGG analysis showed that hub module genes were abundantly expressed in various immune-related aspects, including the response to stimuli, the immune system process, signal transducer activity, autophagy, the NOD-like receptor signaling pathway, the toll-like receptor signaling pathway, the T cell receptor signaling pathway, and Th17 cell differentiation. Additionally, genes related to Th cell differentiation are presented as heatmaps. These genes constitute a complex immune regulatory network, mainly involved in pathogen recognition, antigen processing and presentation, and Th cell differentiation. The results of this study provide the first transcriptome profile of PerCs associated with inactivated immunity and lay a solid foundation for further studies on effective vaccines.
（某种细菌名称）是一种严重危害比目鱼和其他水产养殖物种的细菌。疫苗接种是预防弧菌病的有效手段，主要通过腹腔注射进行。免疫初期有效的抗原处理对于引发适应性免疫反应和提高疫苗效力至关重要。为了解灭活（该细菌）后比目鱼的早期免疫反应，我们检测了灭活（该细菌）免疫后腹腔细胞（PerCs）的转录组图谱。获得了超过100亿条高质量 reads，其中约89.33%成功映射到比目鱼的参考基因组。在免疫后6、12、24和48小时分别捕获了1985、3072、4001和5476个差异表达基因。通过WGCNA鉴定了与免疫时间相关的枢纽模块。GO和KEGG分析表明，枢纽模块基因在各种免疫相关方面大量表达，包括对刺激的反应、免疫系统过程、信号转导活性、自噬、NOD样受体信号通路、Toll样受体信号通路、T细胞受体信号通路和Th17细胞分化。此外，与Th细胞分化相关的基因以热图形式呈现。这些基因构成了一个复杂的免疫调节网络，主要参与病原体识别、抗原处理和呈递以及Th细胞分化。本研究结果提供了与灭活（该细菌）免疫相关的PerCs的首个转录组图谱，为进一步研究有效的（该细菌）疫苗奠定了坚实基础。
