Dahdouh Elias, Lázaro-Perona Fernando, Ruiz-Carrascoso Guillermo, Sánchez García Laura, Saenz de Pipaón Miguel, Mingorance Jesús
Servicio de Microbiología, Hospital Universitario La Paz, IdiPAZ, Paseo de la Castellana, 261, 28046 Madrid, Spain.
Servicio de Neonatología, Hospital Universitario La Paz, 28046 Madrid, Spain.
Microorganisms. 2021 Oct 31;9(11):2271. doi: 10.3390/microorganisms9112271.
(1) Background: We determined the relevance of intestinal dominance by spp. during a neonatal outbreak over 13 weeks. (2) Methods: Rectal swabs (n = 110) were obtained from 42 neonates. spp. was cultured from swabs obtained from 13 neonates (Group 1), while the other 29 neonates were culture-negative (Group 2). Total DNA was extracted from rectal swabs, and quantitative PCRs (qPCRs) using - and -gene-specific primers were performed. relative intestinal loads (RLs) were determined using ΔΔC. Clonality was investigated by random amplified polymorphic DNA analysis and whole-genome sequencing. (3) Results: The outbreak was caused by during the first eight weeks and during the remaining five weeks. spp. were detected by qPCR in all Group 1 neonates and eleven Group 2 neonates. RLs of spp. were higher in Group 1 as compared to Group 2 (6.31% vs. 0.09%, < 0.05) and in the first swab compared to the last (26.9% vs. 4.37%, < 0.05). Nine neonates had extraintestinal detection of spp.; eight of them were infected. RLs of the patients with extraintestinal spread were higher than the rest (2.79% vs. 0.29%, < 0.05). (4) Conclusions: Intestinal dominance by spp. plays a role in outbreaks and extraintestinal spread.
(1) 背景：我们确定了在13周的新生儿疫情期间，[某种细菌名称]属细菌在肠道中的优势地位的相关性。(2) 方法：从42名新生儿中获取直肠拭子（n = 110）。从13名新生儿（第1组）的拭子中培养出了[某种细菌名称]属细菌，而其他29名新生儿培养结果为阴性（第2组）。从直肠拭子中提取总DNA，并使用[基因名称1]和[基因名称2]特异性引物进行定量PCR（qPCR）。使用ΔΔC法确定[某种细菌名称]属细菌的相对肠道负荷（RLs）。通过随机扩增多态性DNA分析和全基因组测序研究克隆性。(3) 结果：疫情在前八周由[某种细菌名称1]引起，在其余五周由[某种细菌名称2]引起。通过qPCR在所有第1组新生儿和11名第2组新生儿中检测到了[某种细菌名称]属细菌。与第2组相比，第1组中[某种细菌名称]属细菌的RLs更高（6.31% 对 0.09%，P < 0.05），并且第一次拭子中的RLs高于最后一次拭子（26.9% 对 4.37%，P < 0.05）。9名新生儿在肠道外检测到了[某种细菌名称]属细菌；其中8名被感染。肠道外传播患者的RLs高于其余患者（2.79% 对 0.29%，P < 0.05）。(4) 结论：[某种细菌名称]属细菌在肠道中的优势地位在疫情和肠道外传播中起作用。
