使用等温微量热法进行检测及药敏试验。
Detection and Drug Susceptibility Testing of Using Isothermal Microcalorimetry.
作者信息
Grütter Anabel E, Lafranca Tecla, Sigg Aurelia Pahnita, Mariotti Max, Bonkat Gernot, Braissant Olivier
机构信息
Department of Biomedical Engineering, University of Basel, Gewerbestrasse 14, 4123 Allschwil, Switzerland.
alta uro AG, Centralbahnplatz 6, 4051 Basel, Switzerland.
出版信息
Microorganisms. 2021 Nov 11;9(11):2337. doi: 10.3390/microorganisms9112337.
BACKGROUND
Gonorrhea is a frequently encountered sexually transmitted disease that results in urethritis and can further lead to pelvic inflammatory disease, infertility, and possibly disseminated gonococcal infections. Thus, it must be diagnosed promptly and accurately. In addition, drug susceptibility testing should be performed rapidly as well. Unfortunately, is a fastidious microorganism that is difficult to grow and requires culturing in an opaque medium.
METHODS
Here, we used isothermal microcalorimetry (IMC) to monitor the growth and the antimicrobial susceptibility of .
RESULTS
Using IMC, concentrations of between 2000 and 1 CFU·mL were detected within 12 to 33 h. In addition, drug susceptibility could be monitored easily.
CONCLUSIONS
The use of isothermal microcalorimetry provides an interesting and useful tool to detect and characterize fastidious microbes such as that require media incompatible with optical detection conventionally used in many commercial systems.
背景
淋病是一种常见的性传播疾病，可导致尿道炎，并可能进一步引发盆腔炎、不孕症以及播散性淋球菌感染。因此，必须及时、准确地进行诊断。此外，药敏试验也应迅速开展。不幸的是，[病原体名称]是一种苛求菌，难以培养，需要在不透明培养基中培养。
方法
在此，我们使用等温微量热法（IMC）监测[病原体名称]的生长及药敏情况。
结果
使用IMC，在12至33小时内检测到[病原体名称]的浓度在2000至1 CFU·mL之间。此外，药敏情况能够轻松监测。
结论
等温微量热法的应用为检测和鉴定苛求微生物（如[病原体名称]）提供了一种有趣且有用的工具，这类微生物需要与许多商业系统中常规使用的光学检测不兼容的培养基。
Zotero 插件