Division of General Science, Faculty of Education, Buriram Rajabhat University, Buriram 31000, Thailand.
Center of Excellence in Cancer Cell and Molecular Biology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Molecules. 2024 May 24;29(11):2473. doi: 10.3390/molecules29112473.
This study aimed to isolate and purify resveratrol and oxyresveratrol from the heartwoods of , and to evaluate their inhibitory effects on melanogenesis in B16F10 murine melanoma cells. A methanol maceration process yielded a crude extract comprising 24.86% of the initial mass, which was subsequently analyzed through HPTLC, HPLC, and LC-MS/MS. These analyses revealed the presence of resveratrol and oxyresveratrol at concentrations of 4.32 mg/g and 33.6 mg/g in the extract, respectively. Initial purification employing food-grade silica gel column chromatography separated the extract into two fractions: FA, exhibiting potent inhibition of both tyrosinase activity and melanogenesis, and FM, showing no such inhibitory activity. Further purification processes led to the isolation of fractions Y11 and Gn12 with enhanced concentrations of resveratrol (94.9 and 110.21 mg/g, respectively) and fractions Gn15 and Gn16 with elevated levels of oxyresveratrol (321.93 and 274.59 mg/g, respectively), all of which significantly reduced melanin synthesis. These outcomes affirm the substantial presence of resveratrol and oxyresveratrol in the heartwood of , indicating their promising role as natural agents for skin lightening.
本研究旨在从心材中分离和纯化白藜芦醇和氧化白藜芦醇，并评估它们对 B16F10 鼠黑素瘤细胞中黑色素生成的抑制作用。甲醇浸提过程得到了一个包含初始质量 24.86%的粗提取物，随后通过 HPTLC、HPLC 和 LC-MS/MS 进行分析。这些分析表明，提取物中分别含有 4.32mg/g 和 33.6mg/g 的白藜芦醇和氧化白藜芦醇。采用食品级硅胶柱色谱法对提取物进行初步纯化，将其分离成两个馏分：FA，对酪氨酸酶活性和黑色素生成均有强烈的抑制作用；FM，无抑制作用。进一步的纯化过程导致分离出的 Y11 和 Gn12 馏分中白藜芦醇的浓度分别提高到 94.9 和 110.21mg/g，Gn15 和 Gn16 馏分中氧化白藜芦醇的浓度分别提高到 321.93 和 274.59mg/g，所有这些馏分都显著降低了黑色素的合成。这些结果证实了心材中白藜芦醇和氧化白藜芦醇的大量存在，表明它们作为皮肤美白的天然物质具有很大的潜力。
