厚朴花提取物对黑色素生成的抑制作用及抗氧化性能。
Inhibition of melanogenesis and antioxidant properties of Magnolia grandiflora L. flower extract.
机构信息
Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan.
出版信息
BMC Complement Altern Med. 2012 Jun 6;12:72. doi: 10.1186/1472-6882-12-72.
BACKGROUND
Magnolia grandiflora L. flower is wildly used in Asian as a traditional herbal medication. The purpose of the study was to investigate the antimelanogenic and antioxidant properties of Magnolia grandiflora L. flower extract. In the study, the inhibitory effects of M. grandiflora L. flower extract on mushroom tyrosinase, B16F10 intracellular tyrosinase activity and melanin content were determined spectrophotometrically. Meanwhile, the antioxidative capacity of the flower extract was also investigated.
RESULTS
Our results revealed that M. grandiflora L. flower extract inhibit mushroom tyrosinase activity (IC(50) = 11.1%; v/v), the flower extract also effectively suppressed intracellular tyrosinase activity (IC(50) = 13.6%; v/v) and decreased the amount of melanin (IC(50) = 25.6%; v/v) in a dose-dependent manner in B16F10 cells. Protein expression level of tyrosinase and tyrosinase-related protein 1 (TRP-1) were also decreased by the flower extract. Additionally, antioxidant capacities such as ABTS(+) free radical scavenging activity, reducing capacity and total phenolic content of the flower extract were increased in a dose-dependent pattern.
CONCLUSIONS
Our results concluded that M. grandiflora L. flower extract decreased the expression of tyrosinase and TRP-1, and then inhibited melanogenesis in B16F10 cells. The flower extract also show antioxidant capacities and depleted cellular reactive oxygen species (ROS). Hence, M. grandiflora L. flower extract could be applied as a type of dermatological whitening agent in skin care products.
背景
玉兰属植物的花朵在亚洲被广泛用作传统草药。本研究旨在探讨玉兰属植物花提取物的抗黑色素生成和抗氧化特性。在研究中，通过分光光度法测定了 M. grandiflora L. 花提取物对蘑菇酪氨酸酶、B16F10 细胞内酪氨酸酶活性和黑色素含量的抑制作用。同时，还研究了花提取物的抗氧化能力。
结果
我们的结果表明，M. grandiflora L. 花提取物抑制蘑菇酪氨酸酶活性（IC50 = 11.1%；v/v），花提取物还能有效抑制细胞内酪氨酸酶活性（IC50 = 13.6%；v/v），并降低 B16F10 细胞中黑色素的含量（IC50 = 25.6%；v/v），呈剂量依赖性。花提取物还降低了酪氨酸酶和酪氨酸酶相关蛋白 1（TRP-1）的蛋白表达水平。此外，花提取物的 ABTS（+）自由基清除活性、还原能力和总酚含量等抗氧化能力呈剂量依赖性增加。
结论
我们的研究结果表明，M. grandiflora L. 花提取物降低了酪氨酸酶和 TRP-1 的表达，从而抑制了 B16F10 细胞中的黑色素生成。花提取物还具有抗氧化能力，并能消耗细胞内的活性氧（ROS）。因此，M. grandiflora L. 花提取物可作为一种皮肤美白剂应用于护肤品中。
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