Brinkman Fiona S L, Winsor Geoffrey L, Done Rachel E, Filloux Alain, Francis Vanessa I, Goldberg Joanna B, Greenberg E Peter, Han Kook, Hancock Robert E W, Haney Cara H, Häußler Susanne, Klockgether Jens, Lamont Iain L, Levesque Roger C, Lory Stephen, Nikel Pablo I, Porter Steven L, Scurlock Matthew W, Schweizer Herbert P, Tümmler Burkhard, Wang Meng, Welch Martin
Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, Canada.
Department of Pediatrics, Division of Pulmonary, Allergy and Immunology, Cystic Fibrosis, and Sleep, Emory Children's Center for Cystic Fibrosis and Airway Disease Research, Emory University School of Medicine, Atlanta, GA, United States.
Adv Microb Physiol. 2021;79:25-88. doi: 10.1016/bs.ampbs.2021.07.001. Epub 2021 Nov 16.
Toward the end of August 2000, the 6.3 Mbp whole genome sequence of Pseudomonas aeruginosa strain PAO1 was published. With 5570 open reading frames (ORFs), PAO1 had the largest microbial genome sequenced up to that point in time-including a large proportion of metabolic, transport and antimicrobial resistance genes supporting its ability to colonize diverse environments. A remarkable 9% of its ORFs were predicted to encode proteins with regulatory functions, providing new insight into bacterial network complexity as a function of network size. In this celebratory article, we fast forward 20 years, and examine how access to this resource has transformed our understanding of P. aeruginosa. What follows is more than a simple review or commentary; we have specifically asked some of the leaders in the field to provide personal reflections on how the PAO1 genome sequence, along with the Pseudomonas Community Annotation Project (PseudoCAP) and Pseudomonas Genome Database (pseudomonas.com), have contributed to the many exciting discoveries in this field. In addition to bringing us all up to date with the latest developments, we also ask our contributors to speculate on how the next 20 years of Pseudomonas research might pan out.
2000年8月底，铜绿假单胞菌PAO1菌株的630万碱基对全基因组序列发表。PAO1有5570个开放阅读框（ORF），是当时已测序的最大微生物基因组，其中很大一部分是代谢、转运和抗微生物耐药基因，这支持了它在不同环境中定殖的能力。其ORF中有9%被预测编码具有调控功能的蛋白质，这为作为网络大小函数的细菌网络复杂性提供了新见解。在这篇纪念文章中，我们将时间快进20年，审视获取这一资源如何改变了我们对铜绿假单胞菌的理解。接下来的内容不仅仅是简单的综述或评论；我们特别邀请了该领域的一些领军人物就PAO1基因组序列以及假单胞菌社区注释项目（PseudoCAP）和假单胞菌基因组数据库（pseudomonas.com）如何推动该领域众多令人兴奋的发现发表个人看法。除了让我们了解最新进展外，我们还请撰稿人推测未来20年假单胞菌研究可能的发展情况。
