Płócienniczak Patrycja, Rębiś Tomasz, Leda Amanda, Milczarek Grzegorz
Institute of Chemistry and Technical Electrochemistry, Poznan University of Technology, Berdychowo 4, 60-965 Poznan, Poland.
Institute of Chemistry and Technical Electrochemistry, Poznan University of Technology, Berdychowo 4, 60-965 Poznan, Poland.
Colloids Surf B Biointerfaces. 2022 Feb;210:112222. doi: 10.1016/j.colsurfb.2021.112222. Epub 2021 Nov 21.
It is presented in this work that lignosulfonate (LS) can be preferentially adsorbed on the surface of multi-walled carbon nanotubes (MWCNT) giving rise to the functional platform for platinum nanoparticles (NPt) deposition. The novel MWCNT/LS/NPt hybrid material has been characterized by X-ray diffraction (XRD), Fourier transformation infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS). The morphology of the MWCNT/LS/NPt electrodes has been investigated by atomic force microscopy (AFM). The electrochemical studies of MWCNT/LS/NPt hybrid material revealed strong electrocatalytic properties towards hydrogen peroxide. In addition, the effects of lignosulfonate amount adsorbed at the MWCNT on the voltammetric response of the hydrogen peroxide were discussed and used to select the optimal and effective conditions for the synthesis of the electrode material. An amperometric biosensor for glucose was developed based on the covalent linkage of glucose oxidase (GOx) at the MWCNT/LS/NPt. The enzyme electrode exhibited excellent electrocatalytic activity and rapid response for glucose. The linear range of the glucose determination was 50-1400 µM and LOD was quantified as 15.67 µM.
本研究表明，木质素磺酸盐（LS）可优先吸附在多壁碳纳米管（MWCNT）表面，形成用于铂纳米颗粒（NPt）沉积的功能平台。通过X射线衍射（XRD）、傅里叶变换红外光谱（FT-IR）、X射线光电子能谱（XPS）对新型MWCNT/LS/NPt杂化材料进行了表征。利用原子力显微镜（AFM）研究了MWCNT/LS/NPt电极的形貌。MWCNT/LS/NPt杂化材料的电化学研究表明其对过氧化氢具有很强的电催化性能。此外，还讨论了吸附在MWCNT上的木质素磺酸盐量对过氧化氢伏安响应的影响，并用于选择合成电极材料的最佳有效条件。基于葡萄糖氧化酶（GOx）在MWCNT/LS/NPt上的共价连接，开发了一种用于葡萄糖的安培生物传感器。该酶电极对葡萄糖表现出优异的电催化活性和快速响应。葡萄糖测定的线性范围为50-1400 μM，检测限为15.67 μM。
