Lignosulfonate-assisted synthesis of platinum nanoparticles deposited on multi-walled carbon nanotubes for biosensing of glucose.

It is presented in this work that lignosulfonate (LS) can be preferentially adsorbed on the surface of multi-walled carbon nanotubes (MWCNT) giving rise to the functional platform for platinum nanoparticles (NPt) deposition. The novel MWCNT/LS/NPt hybrid material has been characterized by X-ray diffraction (XRD), Fourier transformation infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS). The morphology of the MWCNT/LS/NPt electrodes has been investigated by atomic force microscopy (AFM). The electrochemical studies of MWCNT/LS/NPt hybrid material revealed strong electrocatalytic properties towards hydrogen peroxide. In addition, the effects of lignosulfonate amount adsorbed at the MWCNT on the voltammetric response of the hydrogen peroxide were discussed and used to select the optimal and effective conditions for the synthesis of the electrode material. An amperometric biosensor for glucose was developed based on the covalent linkage of glucose oxidase (GOx) at the MWCNT/LS/NPt. The enzyme electrode exhibited excellent electrocatalytic activity and rapid response for glucose. The linear range of the glucose determination was 50-1400 µM and LOD was quantified as 15.67 µM.