Department of Medicinal Chemistry, Collegium Medicum in Bydgoszcz, Faculty of Pharmacy, Nicolaus Copernicus University in Torun, Dr. A. Jurasza 2, 85-089 Bydgoszcz, Poland.
Faculty of Health Sciences, Collegium Medicum, The Mazovian University in Płock, 09-402 Płock, Poland.
Sensors (Basel). 2024 Sep 27;24(19):6257. doi: 10.3390/s24196257.
The demetallation reaction of sulfanyl magnesium(II) porphyrazine with N-ethylphthalimide substituents, followed by remetallation with manganese(II) salts, yields the corresponding manganese(III) derivative (Pz3) with high efficiency. This novel manganese(III) sulfanyl porphyrazine was characterized by HPLC and analyzed using UV-Vis, MS, and FT-IR spectroscopy. Electrochemical experiments of Pz3 conducted in dichloromethane revealed electrochemical activity of the new complex due to both manganese and N-ethylphthalimide substituents redox transitions. Subsequently, Pz3 was deposited on multiwalled carbon nanotubes (MWCNTs), and this hybrid material was then applied to glassy carbon electrodes (GC). The resulting hybrid electroactive electrode material, combining manganese(III) porphyrazine with MWCNTs, showed a significant decrease in overpotential of HO oxidation compared to bare GC or GC electrodes modified with only carbon nanotubes (GC/MWCNTs). This improvement, attributed to the electrocatalytic performance of Mn, enabled linear response and sensitive detection of HO at neutral pH. Furthermore, a glucose oxidase (GOx)-containing biosensing platform was developed by modifying the prepared GC/MWCNT/Pz3 electrode for the electrochemical detection of glucose. The bioelectrode incorporating the newly designed Pz3 exhibited good activity in the presence of glucose, confirming effective electronic communication between the Pz3, GOx and MWCNT surface. The linear range for glucose detection was 0.2-3.7 mM.
具有 N-乙基邻苯二甲酰亚胺取代基的硫代镁(II)卟啉的去金属化反应，随后与锰(II)盐重新金属化，高效地生成相应的锰(III)衍生物 (Pz3)。这种新型的锰(III)硫代卟啉通过 HPLC 进行了表征，并通过 UV-Vis、MS 和 FT-IR 光谱进行了分析。在二氯甲烷中进行的 Pz3 的电化学实验揭示了由于锰和 N-乙基邻苯二甲酰亚胺取代基的氧化还原跃迁，新配合物具有电化学活性。随后，将 Pz3 沉积在多壁碳纳米管 (MWCNTs) 上，并将这种混合材料应用于玻璃碳电极 (GC)。所得的混合电活性电极材料将锰(III)卟啉与 MWCNTs 结合，与裸 GC 或仅用碳纳米管修饰的 GC（GC/MWCNTs）相比，HO 氧化的过电势显著降低。这种改进归因于 Mn 的电催化性能，使 HO 在中性 pH 下具有线性响应和灵敏检测。此外，通过修饰制备的 GC/MWCNT/Pz3 电极，开发了包含葡萄糖氧化酶 (GOx) 的生物传感平台，用于电化学检测葡萄糖。在存在葡萄糖的情况下，包含新设计的 Pz3 的生物电极表现出良好的活性，证实了 Pz3、GOx 和 MWCNT 表面之间的有效电子通信。葡萄糖检测的线性范围为 0.2-3.7 mM。
