回音壁模式生物传感：单分子水平的无标记检测。

Whispering-gallery-mode biosensing: label-free detection down to single molecules.

作者信息

Vollmer Frank, Arnold Stephen

机构信息

The Rowland Institute, Harvard University, 100 Edwin H. Land Blvd., Cambridge, Massachusetts 02142, USA.

出版信息

Nat Methods. 2008 Jul;5(7):591-6. doi: 10.1038/nmeth.1221.

DOI:10.1038/nmeth.1221

PMID:18587317

Abstract

Optical label-free detectors, such as the venerable surface plasmon resonance (SPR) sensor, are generally favored for their ability to obtain quantitative data on intermolecular binding. However, before the recent introduction of resonant microcavities that use whispering gallery mode (WGM) recirculation, sensitivity to single binding events had not materialized. Here we describe the enhancement mechanisms responsible for the extreme sensitivity of the WGM biosensor, review its current implementations and applications, and discuss its future possibilities.

摘要

诸如备受推崇的表面等离子体共振（SPR）传感器之类的无光学标记探测器，通常因其能够获取分子间结合的定量数据而受到青睐。然而，在最近引入利用回音壁模式（WGM）再循环的共振微腔之前，对单个结合事件的灵敏度尚未实现。在这里，我们描述了导致WGM生物传感器具有极高灵敏度的增强机制，回顾了其当前的实现方式和应用，并讨论了其未来的可能性。